Changes in Fatty Acid Levels during In Vitro Ruminal Fluid Incubation with Different Proportions of Maize Distillers Dried Grains (DDGS)

This study aimed to analyse changes in the profile of long-chain fatty acids in the ruminal fluid of cows during in vitro fermentation, using different proportions of maize DDGS (distillers dried grains with solubles) as a substrate. The serum bottles were filled with 1 g of concentrate feed (C), which consisted of cereal middlings, postextraction rapeseed meal, and soybean meal. Substrates I, II, and III contained the same ingredients as substrate C, but also included DDGS at increasing proportions, while substrate IV contained only DDGS. Ruminal fluid with a buffer was then added to the bottles and incubated for 4, 8, and 24 h. After incubation, the fatty acid profile was analysed using a gas chromatograph. The use of DDGS as a substrate resulted in a decrease in SFA, and an increase in the proportion of UFA, including oleic acid (C18:1n9c) and linoleic acid (C18:2n6c). The fermentation profile with 15% and 20% DDGS in TMR proved to be the most beneficial. These findings suggest that the byproduct of bioethanol production could potentially improve the fatty acid profile in the ruminal fluid, resulting in higher-quality animal products.