IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v13y2023i3p660-d1094789.html
   My bibliography  Save this article

Comparative Primary Metabolite Profiling of Setaria viridis Reveals Potential Markers to Water Limitation

Author

Listed:
  • Fernanda Alves de Freitas Guedes

    (Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Prof Rodolpho Paulo Rocco, s/n–CCS, Rio de Janeiro 21941-970, RJ, Brazil
    Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil
    These authors contributed equally to this work.)

  • Luana Beatriz dos Santos Nascimento

    (Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil
    These authors contributed equally to this work.)

  • Mara Priscila Costa

    (Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil)

  • Andrew Macrae

    (Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil
    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, RJ, Brazil)

  • Marcio Alves-Ferreira

    (Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Prof Rodolpho Paulo Rocco, s/n–CCS, Rio de Janeiro 21941-970, RJ, Brazil
    Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil)

  • Camila Caldana

    (National Center for Research in Energy and Materials (CNPEM), Campinas 13083-100, SP, Brazil)

  • Fernanda Reinert

    (Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, s/n–CCS, Rio de Janeiro 21941-590, RJ, Brazil
    Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Prof Rodolpho Paulo Rocco, s/n–CCS, Rio de Janeiro 21941-970, RJ, Brazil)

Abstract

Growing varieties with higher water-use efficiency is crucial to address water limitation in agriculture. Breeding programs often resort to model plants, and Setaria viridis has been consolidating its position as a model for C 4 grasses. However, we lack a detailed analysis of drought-induced metabolic changes in S. viridis . To partially redress this, we assessed the primary metabolic profile of roots, leaves, and panicles in response to three watering levels. Five-day-old seedlings were submitted to water-limiting conditions for 25 days when samples were harvested. GC-MS-based analysis revealed that each plant organ had a specific metabolic profile, with TCA intermediates altered in above- and underground parts. The sPLS-DA analysis allowed clear separation of the water regimes for the three organs. Of the 36 most important metabolites, only four (sucrose, glycerol-3P, gluconate and adenine) were shared by all plant organs. A subset of 12 metabolites, including proline, were further evaluated as drought bioindicator candidates, with galactinol and gluconate emerging for vegetative parts while alanine seems informative of aerial part water status. In general, water limitation decreased the content of nitrogen compounds in aboveground tissues and increased the amounts of carbohydrates, especially in the sink organs. This study adds to our understanding of the metabolic responses of grasses to water limitation and identified potential bioindicators for drought in different plant organs.

Suggested Citation

  • Fernanda Alves de Freitas Guedes & Luana Beatriz dos Santos Nascimento & Mara Priscila Costa & Andrew Macrae & Marcio Alves-Ferreira & Camila Caldana & Fernanda Reinert, 2023. "Comparative Primary Metabolite Profiling of Setaria viridis Reveals Potential Markers to Water Limitation," Agriculture, MDPI, vol. 13(3), pages 1-19, March.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:3:p:660-:d:1094789
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/13/3/660/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/13/3/660/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:13:y:2023:i:3:p:660-:d:1094789. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.