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Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells

Author

Listed:
  • Xin Zhao

    (Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China
    College of Life Science, Nankai University, Tianjin 300071, China
    These authors contributed equally to this work.)

  • Jun Li

    (College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
    These authors contributed equally to this work.)

  • Shuying Zhao

    (College of Animal Science and Animal Medicine, Tianjin Agricultural University, Tianjin 300384, China)

  • Lili Chen

    (Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China)

  • Man Zhang

    (Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China)

  • Yi Ma

    (Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China)

  • Dawei Yao

    (Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China)

Abstract

MicroRNAs (miRNAs) are small RNA molecules consisting of approximately 22 nucleotides that are engaged in the regulation of various bio-processes. There is growing evidence that miR-29 is a key regulator of hepatic lipid metabolism. Mimics and inhibitors of bta-miRNA29d-3p were transiently transfected in bovine mammary epithelial cells (BMECs) to reveal the regulation of bta-miRNA29d-3p on lipid accumulation in BMECs. Results showed that overexpression of bta-miRNA29d-3p significantly inhibited the expression of genes related to triglyceride (TAG) synthesis, namely DGAT1 and mitochondrial glycerol-3-phosphate acyltransferase ( GPAM , p < 0.01) and down-regulated TAG levels in cells ( p < 0.05). The expression of fatty acid synthesis and desaturation-related genes FASN , SCD1 , and ACACA , and transcription factor SREBF1 also decreased. Interference of bta-miRNA29d-3p significantly increased the expression of GPAM , DGAT1 , FASN , SCD1 , ACACA , and SREBF1 ( p < 0.01), and significantly upregulated the concentration of TAG in cells. Furthermore, a luciferase reporter assay confirmed that GPAM is a direct target of bta-miRNA29d-3p. In summary, bta-miRNA29d-3p modulates fatty acid metabolism and TAG synthesis by regulating genes related to lipid metabolism in BMECs and targeting GPAM . Thus, bta-miRNA29d-3p plays an important role in controlling mammary lipid synthesis in cows.

Suggested Citation

  • Xin Zhao & Jun Li & Shuying Zhao & Lili Chen & Man Zhang & Yi Ma & Dawei Yao, 2023. "Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells," Agriculture, MDPI, vol. 13(2), pages 1-10, February.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:2:p:501-:d:1074619
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    Cited by:

    1. Sitian Yang & Xiang Cao & Yu Wang & Cong Li & Zhi Chen, 2023. "Genetics and Production of Safe, High-Quality Milk by Dairy Cattle," Agriculture, MDPI, vol. 13(7), pages 1-3, July.

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