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Use of Gas Chromatography and SPME Extraction for the Differentiation between Healthy and Paenibacillus larvae Infected Colonies of Bee Brood—Preliminary Research

Author

Listed:
  • Bartosz Piechowicz

    (Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland
    Interdisciplinary Center for Preclinical and Clinical Research, University of Rzeszow, Werynia 2, 36-100 Kolbuszowa, Poland)

  • Aleksandra Kwiatek

    (Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland)

  • Stanisław Sadło

    (Department of Ecotoxicology, Faculty of Biotechnology, University of Rzeszów, Rejtana 16c, 35-959 Rzeszow, Poland)

  • Lech Zaręba

    (Interdisciplinary Centre for Computational Modelling, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland)

  • Anna Koziorowska

    (Interdisciplinary Center for Preclinical and Clinical Research, University of Rzeszow, Werynia 2, 36-100 Kolbuszowa, Poland
    Institute of Material Engineering, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland)

  • Daniela Kloc

    (Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland)

  • Maciej Balawejder

    (Institute of Agricultural Sciences, College of Natural Sciences, University of Rzeszow, Zelwerowicza 8b, 35-601 Rzeszow, Poland)

Abstract

Paenibacillus larvae is a deadly pathogen for bee brood, which can lead to the death of entire colonies. The presence of specific volatile organic compounds (VOCs) in the hive may be related to the occurrence of this bacterium in brood. Compositions of those volatile fractions present in healthy brood from control colonies and the brood without symptoms of infection collected from the colonies infected by P. larvae were compared using gas chromatography coupled with mass spectrometry (GC-MS) and solid phase microextraction (SPME). Among the seven compounds detected and quantified, the relative concentrations of 3-carene and limonene significantly differentiated the brood from healthy and infected colonies. Based on the ratio analysis, the samples were differentiated in terms of the number of emitted VOCs.

Suggested Citation

  • Bartosz Piechowicz & Aleksandra Kwiatek & Stanisław Sadło & Lech Zaręba & Anna Koziorowska & Daniela Kloc & Maciej Balawejder, 2023. "Use of Gas Chromatography and SPME Extraction for the Differentiation between Healthy and Paenibacillus larvae Infected Colonies of Bee Brood—Preliminary Research," Agriculture, MDPI, vol. 13(2), pages 1-14, February.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:2:p:487-:d:1072940
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    References listed on IDEAS

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    1. Vasilios Liolios & Dimitrios Kanelis & Chrysoula Tananaki & Maria-Anna Rodopoulou, 2022. "A Comparative Study of Healthy and American Foulbrood-Infected Bee Brood ( Apis mellifera L.) through the Investigation of Volatile Compounds," Agriculture, MDPI, vol. 12(6), pages 1-11, June.
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