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Establishment of Tibetan-Sheep-Specific SNP Genetic Markers

Author

Listed:
  • Benmeng Liang

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China
    These authors contributed equally to this work.)

  • Yuhetian Zhao

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China
    These authors contributed equally to this work.)

  • Yabin Pu

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China)

  • Xiaohong He

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China)

  • Jiangang Han

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China
    Animal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland)

  • Baima Danzeng

    (Nagqu City Animal Husbandry and Veterinary Technology Promotion Station, Nagqu 852000, China)

  • Yuehui Ma

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China)

  • Jianfeng Liu

    (National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China)

  • Lin Jiang

    (National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
    Key Laboratory of Livestock and Poultry Resources (Cattle) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Beijing 100193, China)

Abstract

Tibetan sheep are one of the three major coarse sheep breeds in China, and they possess a long history of formation. However, few studies have been conducted on the identification of Tibetan sheep breeds at the molecular level. In this study, a total of 448 individuals from 24 Tibetan sheep populations in the 5 regions of Tibet, Qinghai, Gansu, Yunnan, and Sichuan were analyzed using the Affymetrix Ovine SNP 600K high-density chip to construct specific single-nucleoside polymorphism (SNP) genetic marker sets of Tibetan sheep breeds. Firstly, the genetic structure analysis showed that Yunnan–Tibetan sheep (NL, Ninglang; JC, Jianchuan), Zuogong (ZG), Heizang (HZ), Gongga (GG,) and Tao sheep (TS) can be clearly distinguished from other Tibetan sheep populations. Next, based on the population differentiation index FST, the PCA and NJ tree results showed that only 60 specific SNPs can successfully separate Tibetan sheep in the Yunnan region from Tibetan sheep in other regions, and the distinguishing effect on Yunnan–Tibetan sheep reached 100%. Using the same method, we found that 4 Tibetan sheep breeds, including Zuogong (ZG, 20 SNPs), Heizang (HZ, 60 SNPs), Gongga (GG, 60 SNPs), and Tao sheep (TS, 30 SNPs), can also be distinguished from other Tibetan sheep populations with only a few SNP loci (20–60), and the distinguishing effect reached 100%. Overall, we successfully obtained a Yunnan region-specific (60 SNPs) genetic marker set and 4 breed-specific SNP genetic marker sets (20–60 SNPs) for the first time for the identification of Tibetan sheep breeds at the molecular level. These made up for the lack of genetic marker sets for the identification of Tibetan sheep breeds, and provided a genomic basis for the scientific classification and accurate identification of livestock and poultry genetic resources on the Qinghai–Tibet Plateau.

Suggested Citation

  • Benmeng Liang & Yuhetian Zhao & Yabin Pu & Xiaohong He & Jiangang Han & Baima Danzeng & Yuehui Ma & Jianfeng Liu & Lin Jiang, 2023. "Establishment of Tibetan-Sheep-Specific SNP Genetic Markers," Agriculture, MDPI, vol. 13(2), pages 1-14, January.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:2:p:322-:d:1049704
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