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VvMYBPA2 Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape

Author

Listed:
  • Changmei Liang

    (College of Information Science and Engineering, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
    These authors contributed equally to this work.)

  • Jianyong Guo

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
    These authors contributed equally to this work.)

  • Mingxiang Chen

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

  • Xuehui Zhang

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

  • Guorong Zhang

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

  • Pengfei Zhang

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

  • Jinjun Liang

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

  • Pengfei Wen

    (College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China)

Abstract

Flavan-3-ols are monomers of Proanthocyanidins (PAs), which are important polyphenolic compounds in grapes. Previous studies had shown that VvMYBPA2 was closely related to grape flavan-3-ol monomers biosynthesis, but its regulatory network is still unclear. Here, we found that the contents of (+)-catechin and (−)-epicatechin, the enzyme activities of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) and the expression of VvANR and VvLAR1 were increased in the VvMYBPA2 overexpression grape leaves compared to the control. It was proved that VvMYBPA2 protein interacted with VvWDR1 and VvWDR1 protein interacted with VvMYC2 by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). The promoters of VvANR and VvLAR1 were bound by VvMYBPA2 using yeast one-hybrid (Y1H) assay. These results suggested that VvMYBPA2 could form a trimeric complex with VvWDR1 and VvMYC2 and jointly regulated the expression of flavan-3-ol monomers related genes VvANR and VvLAR1 , thereby affecting the enzyme activities of ANR and LAR and ultimately regulating the contents of flavan-3-ols.

Suggested Citation

  • Changmei Liang & Jianyong Guo & Mingxiang Chen & Xuehui Zhang & Guorong Zhang & Pengfei Zhang & Jinjun Liang & Pengfei Wen, 2022. "VvMYBPA2 Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape," Agriculture, MDPI, vol. 12(9), pages 1-12, September.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:9:p:1414-:d:909494
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    Keywords

    grape; flavan-3-ols; proanthocyanidins; VvMYBPA2 ; MBW complex;
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