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Transcriptome Sequencing of Agave angustifolia Reveals Conservation and Diversification in the Expression of Cinnamyl Alcohol Dehydrogenase Genes in Agave Species

Author

Listed:
  • Xing Huang

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Bochao Xu

    (School of Life Sciences, Hainan University, Haikou 570228, China)

  • Shibei Tan

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Yanlei Huang

    (College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

  • Jingen Xi

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Xu Qin

    (Guangxi Subtropical Crops Research Institute, Nanning 530001, China)

  • Tao Chen

    (Guangxi Subtropical Crops Research Institute, Nanning 530001, China)

  • Helong Chen

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Xiaohan Yang

    (Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA)

  • Kexian Yi

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
    Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou 571101, China
    Key Laboratory of Low Carbon Green Agriculture in Tropical China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China
    Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, China)

Abstract

Agave angustifolia is an important crassulacean acid metabolism plant species, with wide applications in beverage and sisal fiber production. In this study, we carried out a transcriptome analysis of A . angustifolia leaves, generating a total of 58,482,436 clean reads through Illumina paired-end sequencing. De novo transcriptome assembly generated 67,314 unigenes, with about half of them having homologs in four public databases. In the Nr database, Asparagus officinalis was shown to be most closely related to agave, and the early angiosperm Amborella trichopoda was selected as an outgroup species. We further identified five, two, seven, seven, seven, six and six CAD genes in asparagus, amborella, A. deserti , A. tequilana , A. americana , A. hybrid H11648 and A. angustifolia , respectively. The maximum likelihood phylogenetic tree revealed the species-specific expansion of CAD genes in arabidopsis, rice and agave. The expression analysis indicated the conservatively expressed CAD1 / 2 / 4 / 6 , providing candidate targets for manipulation to improve lignin traits. The species-specific expression of CAD3 / 5 / 7 indicates the existence of different regulatory mechanisms controlling the expression of these genes in agave species. This study presents the first transcriptome dataset of A. angustifolia , facilitating future studies on lignin biosynthesis in agave.

Suggested Citation

  • Xing Huang & Bochao Xu & Shibei Tan & Yanlei Huang & Jingen Xi & Xu Qin & Tao Chen & Helong Chen & Xiaohan Yang & Kexian Yi, 2022. "Transcriptome Sequencing of Agave angustifolia Reveals Conservation and Diversification in the Expression of Cinnamyl Alcohol Dehydrogenase Genes in Agave Species," Agriculture, MDPI, vol. 12(7), pages 1-10, July.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:7:p:1003-:d:860272
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    References listed on IDEAS

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    1. Huaiguo Shang & Na Zhang & Zhouli Xie & Siyu Deng & Licong Yi & Xing Huang, 2021. "Genome-Wide Identification and Expression of the PIN Auxin Efflux Carrier Gene Family in Watermelon ( Citrullus lanatus )," Agriculture, MDPI, vol. 11(5), pages 1-11, May.
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