IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v12y2022i6p834-d835388.html
   My bibliography  Save this article

Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Author

Listed:
  • Vesna Župunski

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Radivoje Jevtić

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Milosav Grčak

    (Department of Plant Protection, Faculty of Agriculture, University of Priština–Kosovska Mitrovica, 38219 Lešak, Serbia)

  • Mirjana Lalošević

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Branka Orbović

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Dalibor Živanov

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Desimir Knežević

    (Department of Plant Protection, Faculty of Agriculture, University of Priština–Kosovska Mitrovica, 38219 Lešak, Serbia)

Abstract

Tracking the distribution of Fusarium species and the detection of changes in toxin production provides epidemiological information that is essential for Fusarium head blight (FHB) management. Members of Fusarium graminearum species complex (FGSC) were characterized using species and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species (wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and intercrop) and chemotype grouping were investigated with multiple correspondence analysis and multiple regression modeling. We found that triticale and oat were more related to isolates classified into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated with F. graminearum s. stricto . Cereal crop species affected the frequencies of F. graminearum s. stricto ( p = 0.003) and F. graminearum s. lato ( p = 0.08) and unidentified isolates with morphological characteristics like those of FGSC members ( p = 0.02). The effectiveness of species-specific primers was 60.3% (Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium -damaged kernel values in the wheat–pea intercropping system indicated that intercropping systems have the potential to control FHB.

Suggested Citation

  • Vesna Župunski & Radivoje Jevtić & Milosav Grčak & Mirjana Lalošević & Branka Orbović & Dalibor Živanov & Desimir Knežević, 2022. "Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems," Agriculture, MDPI, vol. 12(6), pages 1-14, June.
  • Handle: RePEc:gam:jagris:v:12:y:2022:i:6:p:834-:d:835388
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/12/6/834/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/2077-0472/12/6/834/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Drakopoulos, Dimitrios & Kägi, Andreas & Six, Johan & Zorn, Alexander & Wettstein, Felix E. & Bucheli, Thomas D. & Forrer, Hans-Rudolf & Vogelgsang, Susanne, 2021. "The agronomic and economic viability of innovative cropping systems to reduce Fusarium head blight and related mycotoxins in wheat," Agricultural Systems, Elsevier, vol. 192(C).
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Chen, Ning & Li, Xianyue & Šimůnek, Jiří & Shi, Haibin & Zhang, Yuehong & Hu, Qi, 2022. "Quantifying inter-species nitrogen competition in the tomato-corn intercropping system with different spatial arrangements," Agricultural Systems, Elsevier, vol. 201(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:12:y:2022:i:6:p:834-:d:835388. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.