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Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice

Author

Listed:
  • Jian Wang

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)

  • Yuexuan Cao

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)

  • Kejian Wang

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)

  • Chaolei Liu

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)

Abstract

In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl haploid inducer lines promises to transform modern rice ( Oryza sativa ) breeding. However, this technology is limited, as haploid inducers and pollen acceptors have asynchronous heading dates. To address this obstacle, we developed a panel of multiple-heading-date mtl haploid inducer lines that produce pollen for more than 35 days. We edited the MTL gene in a hybrid rice with the CRISPR-Cas9 system. We then selected transgene-free homozygous mutants in the T 1 generation and reproduced to T 4 generation by single-seed descent method. We obtained 547 mtl haploid inducers with diverse heading dates (from 73 to 110 days) and selected 16 lines comprising a core population with continuous flowering. The seed-setting rate and haploid induction rate (HIR) of the core panel were 4.0–12.7% and 2.8–12.0%, respectively. Thus, our strategy of using multiple-heading-date mtl haploid inducers could accelerate the use of in vivo DH technology in rice breeding.

Suggested Citation

  • Jian Wang & Yuexuan Cao & Kejian Wang & Chaolei Liu, 2022. "Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice," Agriculture, MDPI, vol. 12(6), pages 1-9, June.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:6:p:806-:d:830553
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