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In Vitro Application of Exogenous Fibrolytic Enzymes from Trichoderma Spp. to Improve Feed Utilization by Ruminants

Author

Listed:
  • Francesco Iannaccone

    (Department of Agricultural and Environmental Science, University of Bari “Aldo Moro”, 70126 Bary, Italy)

  • Vittoria Alborino

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Irene Dini

    (Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy)

  • Anna Balestrieri

    (Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, Italy)

  • Roberta Marra

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
    BAT Center—Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Portici, Italy)

  • Rosario Davino

    (Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80138 Naples, Italy)

  • Antonio Di Francia

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Felicia Masucci

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Francesco Serrapica

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Francesco Vinale

    (BAT Center—Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Portici, Italy
    Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80138 Naples, Italy)

Abstract

Treating ruminant feeds with exogenous fibrolytic enzymes may potentially increase forage cell wall degradability and thus feed efficiency. In nature, fungi biosynthesize lignocellulolytic enzymes that can break down lignocellulosic material into its sugar components, thereby providing ready fermentable substrates. This work showed the in vitro fibrolytic activity of three Trichoderma strains (T. atroviride strain P1, T. afroharzianum strain T22, T. reesei strain T67). Total protein concentration and enzymatic (e.g., glucanase, cellulase, and xylanase) activities were determined in fungal culture filtrates after 7 and 14 days of growth on different fiber-based media. The enzymatic mixtures produced by Trichoderma spp. showed the highest concentration of fibrolytic enzymes and were added to industrial feed to test their ability to hydrolyze insoluble fibers. The supplementation of industrial feeds containing medium-fiber or low-fiber concentrates with T22 enzymes produced in the presence of lyophilized mushrooms and durum wheat fiber reduced hemicellulose concentration up to 33% and 24%, respectively. These results may offer novel opportunities to develop livestock feeds with improved fiber digestibility.

Suggested Citation

  • Francesco Iannaccone & Vittoria Alborino & Irene Dini & Anna Balestrieri & Roberta Marra & Rosario Davino & Antonio Di Francia & Felicia Masucci & Francesco Serrapica & Francesco Vinale, 2022. "In Vitro Application of Exogenous Fibrolytic Enzymes from Trichoderma Spp. to Improve Feed Utilization by Ruminants," Agriculture, MDPI, vol. 12(5), pages 1-16, April.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:5:p:573-:d:797224
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