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Cytpchrome P450 CYP4G68 Is Associated with Imidacloprid and Thiamethoxam Resistance in Field Whitefly, Bemisia tabaci (Hemiptera: Gennadius)

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  • Jinjin Liang

    (College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
    Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Jing Yang

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Jinyu Hu

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Buli Fu

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    The Ministry of Agriculture and Rural Affairs Key Laboratory of Integrated Pest Management of Tropical Crops, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Peipan Gong

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Tianhua Du

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Hu Xue

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Xuegao Wei

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Shaonan Liu

    (College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
    Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Mingjiao Huang

    (College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
    Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Cheng Yin

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Yao Ji

    (College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
    Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Chao He

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Wen Xie

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    National Research Institute of Breeding in Hainan, Chinese Academy of Agricultural Sciences, Sanya 572024, China)

  • Ran Wang

    (Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China)

  • Xin Yang

    (Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    National Research Institute of Breeding in Hainan, Chinese Academy of Agricultural Sciences, Sanya 572024, China)

  • Youjun Zhang

    (College of Plant Protection, Hunan Agricultural University, Changsha 410125, China)

Abstract

The superfamily cytochrome P450s is involved in the evolution of insecticide resistance. However, whether CYP4G68 , a differentially expressed gene identified from our transcriptomics analysis, confers resistance to the world’s heavily used insecticide class neonicotinoids is unknown. Hence, we explored the role of CYP4G68 in conferring imidacloprid and thiamethoxam resistance in Bemisia tabaci . The species B. tabaci MED developed low-to-high resistance to imidacloprid and thiamethoxam. Exposure to imidacloprid and thiamethoxam significantly increased the expression of CYP4G68 . Moreover, quantitative real-time PCR analysis demonstrated that CYP4G68 was remarkably overexpressed in imidacloprid-resistant and thiamethoxam-resistant strains compared to susceptible strains. Further correlation analysis showed that CYP4G68 expression was significantly positively correlated with the associated resistance level in various strains of B. tabaci. These results suggest that the enhanced expression of CYP4G68 appears to mediate imidacloprid and thiamethoxam resistance in B. tabaci . Additionally, silencing CYP4G68 via RNA interference strongly increased the susceptibility of B. tabaci MED to imidacloprid and thiamethoxam. Collectively, this work revealed that CYP4G68 plays a vital role in imidacloprid and thiamethoxam resistance in B. tabaci MED. These findings will not only advance our understanding of the role of P450s in insecticide resistance but also provide a great potential target for the sustainable control of destructive insect pests such as whiteflies.

Suggested Citation

  • Jinjin Liang & Jing Yang & Jinyu Hu & Buli Fu & Peipan Gong & Tianhua Du & Hu Xue & Xuegao Wei & Shaonan Liu & Mingjiao Huang & Cheng Yin & Yao Ji & Chao He & Wen Xie & Ran Wang & Xin Yang & Youjun Zh, 2022. "Cytpchrome P450 CYP4G68 Is Associated with Imidacloprid and Thiamethoxam Resistance in Field Whitefly, Bemisia tabaci (Hemiptera: Gennadius)," Agriculture, MDPI, vol. 12(4), pages 1-17, March.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:4:p:473-:d:780788
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    Cited by:

    1. Xiao Ran & Hadiatullah Hadiatullah & Zhiguang Yuchi & Xin Yang & Xun Zhu, 2023. "Sustainable Use of Pesticides," Agriculture, MDPI, vol. 13(7), pages 1-3, July.

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