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Genome-Wide Association Study Uncovers Loci and Candidate Genes Underlying Phytosterol Variation in Sesame ( Sesamum indicum L.)

Author

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  • Zhijian Wang

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Qi Zhou

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Senouwa Segla Koffi Dossou

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
    Laboratoire de Physiologie et de Biotechnologie Végétales, Faculté des Sciences, Université de Lomé, Lomé 01BP1515, Togo)

  • Rong Zhou

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Yingzhong Zhao

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Wangyi Zhou

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Yanxin Zhang

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Donghua Li

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Jun You

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

  • Linhai Wang

    (Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

Abstract

Sesame is one of the most important oilseed crops grown worldwide. It provides diverse nutraceuticals—including lignans, unsaturated fatty acids (UFA), phytosterols, etc.—to humans. Among sesame’s nutraceuticals, phytosterols have received less attention from sesame breeders, although their biological and pharmacological functions have been recorded. Therefore, in the present study, we evaluated the variation of phytosterol contents in 402 sesame accessions grown in two environments and revealed their associated loci and candidate genes. Gas chromatography (GC) analysis unveiled that sesame mainly contains four phytosterols: campesterol, stigmasterol, β-sitosterol, and Δ5-avenasterol. β-sitosterol (1.6–4.656 mg/g) was the major phytosterol, followed by campesterol (0–2.847 mg/g), stigmasterol (0.356–1.826 mg/g), and Δ5-avenasterol (0–1.307 mg/g). The total phytosterol content varied from 2.694 to 8.388 mg/g. Genome-wide association study identified 33 significant associated single nucleotide polymorphism (SNP) loci for the four traits, of which Ch6-39270 and Ch11-142842 were environmentally stable and simultaneously linked with campesterol and stigmasterol content variation. Candidate genes screening indicated that SINPZ1100015 encoding a NAC domain-containing protein 43 is likely the major candidate effect gene of phytosterol variation in sesame. The results of this study extend knowledge of phytosterol variation in sesame and provide important resources for markers-assisted breeding of high-phytosterol content varieties.

Suggested Citation

  • Zhijian Wang & Qi Zhou & Senouwa Segla Koffi Dossou & Rong Zhou & Yingzhong Zhao & Wangyi Zhou & Yanxin Zhang & Donghua Li & Jun You & Linhai Wang, 2022. "Genome-Wide Association Study Uncovers Loci and Candidate Genes Underlying Phytosterol Variation in Sesame ( Sesamum indicum L.)," Agriculture, MDPI, vol. 12(3), pages 1-13, March.
  • Handle: RePEc:gam:jagris:v:12:y:2022:i:3:p:392-:d:768872
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    References listed on IDEAS

    as
    1. Chengqi Cui & Yanyang Liu & Yan Liu & Xianghua Cui & Zhiyu Sun & Zhenwei Du & Ke Wu & Xiaolin Jiang & Hongxian Mei & Yongzhan Zheng, 2021. "Genome-wide association study of seed coat color in sesame (Sesamum indicum L.)," PLOS ONE, Public Library of Science, vol. 16(5), pages 1-14, May.
    2. Linhui Yu & Jilian Fan & Chao Zhou & Changcheng Xu, 2021. "Sterols are required for the coordinated assembly of lipid droplets in developing seeds," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
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    Cited by:

    1. Baoshan Wang & Qingxi Liao & Lei Wang & Caixia Shu & Mei Cao & Wenbin Du, 2023. "Design and Test of Air-Assisted Seed-Guiding Device of Precision Hill-Seeding Centralized Seed-Metering Device for Sesame," Agriculture, MDPI, vol. 13(2), pages 1-21, February.

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      Keywords

      sesame; plant sterols; GWAS; SNP loci; NAC protein; SiNST1;
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