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Efficacy of Bacillus subtilis XZ18-3 as a Biocontrol Agent against Rhizoctonia cerealis on Wheat

Author

Listed:
  • Yanjie Yi

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

  • Pengyu Luan

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

  • Shifei Liu

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)

  • Youtian Shan

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

  • Zhipeng Hou

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)

  • Shuyun Zhao

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

  • Shao Jia

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

  • Ruifang Li

    (School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
    The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China
    Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou 450001, China)

Abstract

Rhizoctonia cerealis is a major fungal pathogen of wheat that causes great yield losses in all wheat-growing regions of the world. The biocontrol agent Bacillus subtilis XZ18-3 was investigated for inhibiting R. cerealis growth in wheat. The results of the mycelial growth test showed that the sterile filtrate of B . subtilis XZ18-3 could significantly inhibit the mycelial growth of R. cerealis and cause swelling and rupture of the mycelium. Observation by transmission electron microscopy indicated that the sterile filtrate could penetrate the cellular membrane of Rhizoctonia cerealis , resulting in organelle destruction. The effect of the sterile filtrates on the pathogen cells, shown through fluorescent microscopy using different stains, revealed the mechanism by which the sterile filtrate caused DNA fragmentation, accumulation of ROS and changes in cell membrane permeability. To reach a better treatment of the soil-borne fungi, the components of a wettable powder were screened and an optimised formula determined (30.0% kaolin, 4.0% polyvinyl alcohol, 8.0% Tween-80, 2.0% polyethylene glycol and 100% fermentation broth). A quality index analysis revealed that the wetting powder reached acceptable biological pesticide standards. Pot control experiments showed that the wettable powder of B . subtilis XZ18-3 effectively controlled the pathogens with an efficacy of 88.28%. This study has provided the potential biocontrol agents (BCAs) for wheat sharp eyespot disease.

Suggested Citation

  • Yanjie Yi & Pengyu Luan & Shifei Liu & Youtian Shan & Zhipeng Hou & Shuyun Zhao & Shao Jia & Ruifang Li, 2022. "Efficacy of Bacillus subtilis XZ18-3 as a Biocontrol Agent against Rhizoctonia cerealis on Wheat," Agriculture, MDPI, vol. 12(2), pages 1-15, February.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:2:p:258-:d:746912
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    References listed on IDEAS

    as
    1. Jie Liu & Yue He & Shaohua Chen & Ying Xiao & Meiying Hu & Guohua Zhong, 2014. "Development of a Freeze-Dried Fungal Wettable Powder Preparation Able to Biodegrade Chlorpyrifos on Vegetables," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-9, July.
    2. Yueju Zhao & Jonathan Nimal Selvaraj & Fuguo Xing & Lu Zhou & Yan Wang & Huimin Song & Xinxin Tan & Lichao Sun & Lancine Sangare & Yawa Minnie Elodie Folly & Yang Liu, 2014. "Antagonistic Action of Bacillus subtilis Strain SG6 on Fusarium graminearum," PLOS ONE, Public Library of Science, vol. 9(3), pages 1-11, March.
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