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A Single Nucleotide Polymorphism in the WIF1 Promoter Region Regulates the Wool Length in Rabbits

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  • Jiawei Cai

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Bohao Zhao

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Jiali Li

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Zhiyuan Bao

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Yang Chen

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Yan Liu

    (Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China)

  • Xinsheng Wu

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
    Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China)

Abstract

Wool length is an important trait in the rabbit wool industry. Wnt inhibitory factor 1 ( WIF1 ), a key gene, regulates hair follicle growth and development. This study explores the WIF1 gene relationship with wool production traits in three different rabbit breeds, Rex rabbit, New Zealand rabbit, and Angora rabbit. The rabbit WIF1 gene sequence was obtained from GenBank, and PCR and Sanger sequencing were applied to screen SNP (single nucleotide polymorphism) from the gene exons and promoter regions. Sequencing results found no SNP in the PCR products of 10 exons from the rabbit WIF1 gene, indicating that the WIF1 gene was not polymorphic. Thus, the gene was conserved in all three rabbit breeds. Only one SNP was found in the WIF1 promoter region, associated with wool-producing traits in rabbits. Luciferase activity assays showed that the AA genotype had higher transcriptional activities than the AG or GG genotype ( p < 0.01). In addition, WIF1 was significantly upregulated in the Rex rabbit compared to the New Zealand rabbit and Angora rabbit ( p < 0.01). In summary, a key SNP in the WIF1 promoter region was found to be associated with rabbit fiber length trait.

Suggested Citation

  • Jiawei Cai & Bohao Zhao & Jiali Li & Zhiyuan Bao & Yang Chen & Yan Liu & Xinsheng Wu, 2022. "A Single Nucleotide Polymorphism in the WIF1 Promoter Region Regulates the Wool Length in Rabbits," Agriculture, MDPI, vol. 12(11), pages 1-9, November.
  • Handle: RePEc:gam:jagris:v:12:y:2022:i:11:p:1858-:d:964201
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    References listed on IDEAS

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    1. Jen-Chih Hsieh & Laurent Kodjabachian & Martha L. Rebbert & Amir Rattner & Philip M. Smallwood & Cynthia Harryman Samos & Roel Nusse & Igor B. Dawid & Jeremy Nathans, 1999. "A new secreted protein that binds to Wnt proteins and inhibits their activites," Nature, Nature, vol. 398(6726), pages 431-436, April.
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    More about this item

    Keywords

    WIF1 gene; rabbit; fiber length; SNP; promoter activity;
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