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Broad-Spectrum Resistance to Leaf Rust in the Argentinean Wheat Cultivar “Klein Proteo” Is Controlled by LrKP Located on Chromosome 2BS

Author

Listed:
  • Shengsheng Bai

    (School of Advanced Agriculture Sciences, Peking University, Beijing 100871, China
    Institute of Advanced Agricultural Sciences, Peking University, Weifang 261000, China
    These authors contributed equally to this work.)

  • Shuyong Pang

    (State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, China
    Institute of Advanced Agricultural Sciences, Peking University, Weifang 261000, China
    These authors contributed equally to this work.)

  • Hongna Li

    (Institute of Advanced Agricultural Sciences, Peking University, Weifang 261000, China)

  • Jinwei Yang

    (Institute of Advanced Agricultural Sciences, Peking University, Weifang 261000, China)

  • Haitao Yu

    (College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China)

  • Shisheng Chen

    (School of Advanced Agriculture Sciences, Peking University, Beijing 100871, China
    Institute of Advanced Agricultural Sciences, Peking University, Weifang 261000, China)

  • Xiaodong Wang

    (State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, China)

Abstract

Wheat leaf rust, caused by Puccinia triticina , is a severe fungal disease threatening global wheat production. The rational application of genetic loci controlling wheat resistance to leaf rust in breeding practice is still the best choice for disease control. A previous study indicated that the Argentinean wheat cultivar “Klein Proteo” might carry leaf rust resistance ( Lr ) genes Lr3a and Lr10 , as well as an unknown Lr gene. In this study, seedlings of “Klein Proteo” showed high resistance to all the 20 Pt pathotypes isolated in China. Using bulked segregant RNA sequencing (BSR-seq) and developed CAPS markers, the single-dominant gene LrKP was initially mapped to a 114–168 Mb region on chromosome 2BS. Using gene-specific primers of a previously cloned chromosome 2BS-located Lr13 gene, we found that “Klein Proteo” also carried the Lr13 gene. Moreover, the expression of Lr13 in the resistant bulk was significantly higher than that in the susceptible bulk. Nevertheless, “Klein Proteo” showed a much broader and higher resistance compared with the near isogenic line and “ZhouMai 22” carrying Lr13 . In conclusion, the wheat cultivar “Klein Proteo” showed great potential in the genetic improvement of wheat resistance to leaf rust in China and the genetic bases controlling the broad-spectrum resistance were initially revealed.

Suggested Citation

  • Shengsheng Bai & Shuyong Pang & Hongna Li & Jinwei Yang & Haitao Yu & Shisheng Chen & Xiaodong Wang, 2022. "Broad-Spectrum Resistance to Leaf Rust in the Argentinean Wheat Cultivar “Klein Proteo” Is Controlled by LrKP Located on Chromosome 2BS," Agriculture, MDPI, vol. 12(11), pages 1-10, November.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:11:p:1836-:d:961080
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