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The Detection of Wolbachia in Tea Green Leafhopper ( Empoasca onukii Matsuda) and Its Influence on the Host

Author

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  • Qiuqiu Zhang

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

  • Rongmeng Lan

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

  • Dezhong Ji

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

  • Yanni Tan

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

  • Xia Zhou

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

  • Xiaofeng Tan

    (Plant Protection and Quarantine Station of Guizhou Province, Guiyang 550001, China)

  • Qiong Wu

    (Plant Protection and Quarantine Station of Guizhou Province, Guiyang 550001, China)

  • Linhong Jin

    (State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China)

Abstract

Tea green leafhopper ( Empoasca onukii Matsuda) is a critical pest in tea production. Wolbachia has attracted much attention as a new direction of pest biological control for its ability of manipulating the hosts’ reproductive biology. In this work, we focused on the detection of Wolbachia in tea green leafhopper and its effect on host reproduction and development. Polymerase chain reaction (PCR), real-time PCR, and fluorescence in situ hybridization (FISH) techniques were used to detect the distribution of Wolbachia in tea green leafhopper. Wolbachia infection levels were different in different organs of hosts in different insect stages. In addition, comparison between the infected populations and cured population (treated by tetracyclines) revealed that presence of Wolbachia apparently influenced the growth, life cycle, and other reproductive factors of tea green leafhopper, caused, for example, by cytoplasmic incompatibility (CI), thereby reducing number of offspring, shortening lifespan, and causing female-biased sex ratio. This research confirmed that the bacteria Wolbachia was of high incidence in tea leafhoppers and could significantly affect the hosts’ reproductive development and evolution.

Suggested Citation

  • Qiuqiu Zhang & Rongmeng Lan & Dezhong Ji & Yanni Tan & Xia Zhou & Xiaofeng Tan & Qiong Wu & Linhong Jin, 2021. "The Detection of Wolbachia in Tea Green Leafhopper ( Empoasca onukii Matsuda) and Its Influence on the Host," Agriculture, MDPI, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:gam:jagris:v:12:y:2021:i:1:p:36-:d:713468
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