IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v11y2021i2p125-d493068.html
   My bibliography  Save this article

Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)

Author

Listed:
  • Monier M. Abd El-Ghani

    (Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt)

  • Ashraf S. A. El-Sayed

    (Department of Botany and Microbiology, Faculty of Science, Zagazig University, Al Sharqiya Governorate 44519, Egypt)

  • Ahmed Moubarak

    (Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt)

  • Rabab Rashad

    (Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt)

  • Hala Nosier

    (Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt)

  • Adel Khattab

    (Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt)

Abstract

Astragalus L. is one of the largest angiosperm complex genera that belongs to the family Fabaceae, subfamily Papilionoideae or Faboideae under the subtribe Astragalinae of the tribe Galegeae. The current study includes the whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species of Astragalus were collected from different localities in Egypt during the period from February 2018 to May 2019. Morphologically, identification and classification of collected Astragalus plants occurred by utilizing the light microscope, regarding the taxonomic revisions of the reference collected Astragalus specimens in other Egyptian Herbaria. For molecular validation, ten SCoT primers were used in this study, producing a unique banding pattern to differentiate between ten samples of Astragalus taxa which generated 212 DNA fragments with an average of 12.2 bands per 10 Astragalus samples, with 8 to 37 fragments per primer. The 212 fragments amplified were distributed as 2 monomorphic bands, 27 polymorphic without unique bands, 183 unique bands (210 Polymorphic with unique bands), and ITS2 gene sequence was showed as the optimal barcode for identifying Astragalus L. using BLAST searched on NCBI database, and afterward, analyzing the chromatogram for ITS region, 10 samples have been identified as two samples representing A. hauarensis , four samples representing A. sieberi , three samples representing A. spinosus and one sample representing A. vogelii . Based on the ITS barcode, A. hauarensis RMG1, A. hauarensis RMG2, A. sieberi RMG1, A. sieberi RMG2, A. sieberi RMG3, A. sieberi RMG4, A. spinosus RMG1, A. spinosus RMG2, A. spinosus RMG3, A. vogelii RMG were deposited into GenBank with accession # MT367587.1, MT367591.1, MT367593.1, MT367585.1, MT367586.1, MT367588.1, MT160347.1, MT367590.1, MT367589.1, MT367592.1, respectively. These results indicated the efficiency of SCoT markers and ITS2 region in identifying and determining genetic relationships between Astragalus species.

Suggested Citation

  • Monier M. Abd El-Ghani & Ashraf S. A. El-Sayed & Ahmed Moubarak & Rabab Rashad & Hala Nosier & Adel Khattab, 2021. "Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)," Agriculture, MDPI, vol. 11(2), pages 1-16, February.
    • Handle: RePEc:gam:jagris:v:11:y:2021:i:2:p:125-:d:493068
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/11/2/125/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/11/2/125/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:11:y:2021:i:2:p:125-:d:493068. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.