IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v11y2021i1p64-d480403.html
   My bibliography  Save this article

Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Author

Listed:
  • Priyanka Dwivedi

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
    Amity Institute of Organic Agriculture (AIOA), Amity University, Noida 201303, Uttar Pradesh, India)

  • Naleeni Ramawat

    (Amity Institute of Organic Agriculture (AIOA), Amity University, Noida 201303, Uttar Pradesh, India)

  • Gaurav Dhawan

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Subbaiyan Gopala Krishnan

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Kunnummal Kurungara Vinod

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Madan Pal Singh

    (Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Mariappan Nagarajan

    (Rice Breeding and Genetics Research Centre, ICAR- Indian Agricultural Research Institute, Aduthurai 612 101, Tamil Nadu, India)

  • Prolay Kumar Bhowmick

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Nimai Prasad Mandal

    (Central Rainfed Upland Rice Research Station, ICAR-National Rice Research Institute, Post Box 48, Hazaribagh 825 301, Jharkhand, India)

  • Puvvada Perraju

    (College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Rewa 486 004, Madhya Pradesh, India)

  • Haritha Bollinedi

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Ranjith Kumar Ellur

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

  • Ashok Kumar Singh

    (Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India)

Abstract

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1 , governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1 . Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

Suggested Citation

  • Priyanka Dwivedi & Naleeni Ramawat & Gaurav Dhawan & Subbaiyan Gopala Krishnan & Kunnummal Kurungara Vinod & Madan Pal Singh & Mariappan Nagarajan & Prolay Kumar Bhowmick & Nimai Prasad Mandal & Puvva, 2021. "Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress," Agriculture, MDPI, vol. 11(1), pages 1-20, January.
    • Handle: RePEc:gam:jagris:v:11:y:2021:i:1:p:64-:d:480403
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/11/1/64/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/11/1/64/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Joshi, K. & Joshi, P.K. & Khan, M.T. & Kishore, A., 2018. "Sticky Rice': Variety Inertia in a Technologically Progressive State of India," 2018 Conference, July 28-August 2, 2018, Vancouver, British Columbia 277529, International Association of Agricultural Economists.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Shubham Joshi & Viswanathan Chinnusamy & Rohit Joshi, 2022. "Root System Architecture and Omics Approaches for Belowground Abiotic Stress Tolerance in Plants," Agriculture, MDPI, vol. 12(10), pages 1-18, October.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:11:y:2021:i:1:p:64-:d:480403. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.