IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v10y2020i11p505-d436113.html
   My bibliography  Save this article

The In-Silico Development of DNA Markers for Breeding of Spring Barley Varieties That Are Resistant to Spot Blotch in Russia

Author

Listed:
  • Irina V. Rozanova

    (N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia
    Institute of Cytology and Genetics, Siberian Branch of the Russian, Academy of Sciences, 630090 Novosibirsk, Russia)

  • Nina M. Lashina

    (All-Russian Research Institute for Plant Protection, 196608 St. Petersburg, Russia)

  • Vadim M. Efimov

    (Institute of Cytology and Genetics, Siberian Branch of the Russian, Academy of Sciences, 630090 Novosibirsk, Russia
    Computational Genomics Laboratory, Department of Natural Sciences, Novosibirsk State University, Pirogova, 1, 630090 Novosibirsk, Russia)

  • Olga S. Afanasenko

    (All-Russian Research Institute for Plant Protection, 196608 St. Petersburg, Russia)

  • Elena K. Khlestkina

    (N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia
    Institute of Cytology and Genetics, Siberian Branch of the Russian, Academy of Sciences, 630090 Novosibirsk, Russia
    Computational Genomics Laboratory, Department of Natural Sciences, Novosibirsk State University, Pirogova, 1, 630090 Novosibirsk, Russia)

Abstract

The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%. The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen-resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated. Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker-assisted selection.

Suggested Citation

  • Irina V. Rozanova & Nina M. Lashina & Vadim M. Efimov & Olga S. Afanasenko & Elena K. Khlestkina, 2020. "The In-Silico Development of DNA Markers for Breeding of Spring Barley Varieties That Are Resistant to Spot Blotch in Russia," Agriculture, MDPI, vol. 10(11), pages 1-15, October.
    • Handle: RePEc:gam:jagris:v:10:y:2020:i:11:p:505-:d:436113
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/10/11/505/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/10/11/505/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:10:y:2020:i:11:p:505-:d:436113. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.