IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v83y2015icp345-351.html
   My bibliography  Save this article

Escherichia coli hydrogen gas production from glycerol: Effects of external formate

Author

Listed:
  • Trchounian, Karen
  • Trchounian, Armen

Abstract

Hydrogen gas (H2) production was studied in bacteria cultured at different pH conditions, and on different carbon substrates. H2 production by Escherichia coli was first studied during glycerol fermentation when external formate (10 mM) was supplemented. Changes in H2 production rate (VH2) were determined when in the assays glycerol and/or formate were supplemented at pH 7.5 and pH 6.5, using single and double mutants coding large subunits of different hydrogenases (Hyd-1, Hyd-2 and Hyd-3) as well as selC (coding for formate dehydrogenases), hyaB hybC (coding for large subunits of Hyd-1 and Hyd-2, respectively) selC or hyaB hybC hycE (coding for large subunit of Hyd-3) triple mutants at both pHs. The results point out that Hyd-3 becomes mainly responsible for H2 production by E. coli during glycerol fermentation when external formate is added; Hyd-4 can also contribute to H2 production. Besides, in the glycerol supplemented assays, three hydrogenases can work in H2 producing mode and only deletion of three of them decreases the production of H2 which might be due to disturbance of H2 cycling. This is of significance in application of different carbon sources in renewable energy production technology using bacteria.

Suggested Citation

  • Trchounian, Karen & Trchounian, Armen, 2015. "Escherichia coli hydrogen gas production from glycerol: Effects of external formate," Renewable Energy, Elsevier, vol. 83(C), pages 345-351.
  • Handle: RePEc:eee:renene:v:83:y:2015:i:c:p:345-351
    DOI: 10.1016/j.renene.2015.04.052
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148115003328
    Download Restriction: Full text for ScienceDirect subscribers only

    File URL: https://libkey.io/10.1016/j.renene.2015.04.052?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Gabrielyan, Lilit & Sargsyan, Harutyun & Hakobyan, Lilit & Trchounian, Armen, 2014. "Regulation of hydrogen photoproduction in Rhodobacter sphaeroides batch culture by external oxidizers and reducers," Applied Energy, Elsevier, vol. 131(C), pages 20-25.
    2. Yun Jae Kim & Hyun Sook Lee & Eun Sook Kim & Seung Seob Bae & Jae Kyu Lim & Rie Matsumi & Alexander V. Lebedinsky & Tatyana G. Sokolova & Darya A. Kozhevnikova & Sun-Shin Cha & Sang-Jin Kim & Kae Kyou, 2010. "Formate-driven growth coupled with H2 production," Nature, Nature, vol. 467(7313), pages 352-355, September.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Trchounian, Karen & Sawers, R. Gary & Trchounian, Armen, 2017. "Improving biohydrogen productivity by microbial dark- and photo-fermentations: Novel data and future approaches," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 1201-1216.
    2. Trchounian, Karen & Trchounian, Armen, 2015. "Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives," Applied Energy, Elsevier, vol. 156(C), pages 174-184.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ralf Steinhilper & Gabriele Höff & Johann Heider & Bonnie J. Murphy, 2022. "Structure of the membrane-bound formate hydrogenlyase complex from Escherichia coli," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Trchounian, Karen & Poladyan, Anna & Trchounian, Armen, 2016. "Optimizing strategy for Escherichia coli growth and hydrogen production during glycerol fermentation in batch culture: Effects of some heavy metal ions and their mixtures," Applied Energy, Elsevier, vol. 177(C), pages 335-340.
    3. Liu, Wei & Pang, Jian & Wu, Dong & Zhang, Le & Xing, Dexun & Hu, Jianhua & Li, Yongli & Liu, Zhanying, 2023. "Hydrogen production by a novel Klebsiella pneumoniae strain from sheep rumen uses corn straw as substrate," Energy, Elsevier, vol. 282(C).
    4. Trchounian, Karen & Trchounian, Armen, 2015. "Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives," Applied Energy, Elsevier, vol. 156(C), pages 174-184.
    5. Poladyan, Anna & Trchounian, Karen & Vassilian, Anait & Trchounian, Armen, 2018. "Hydrogen production by Escherichia coli using brewery waste: Optimal pretreatment of waste and role of different hydrogenases," Renewable Energy, Elsevier, vol. 115(C), pages 931-936.
    6. Trchounian, Karen & Sawers, R. Gary & Trchounian, Armen, 2017. "Improving biohydrogen productivity by microbial dark- and photo-fermentations: Novel data and future approaches," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 1201-1216.

    More about this item

    Keywords

    Escherichia coli; H2 energy; Hydrogenases; Glycerol fermentation; External formate; pH;
    All these keywords.

    JEL classification:

    • H2 - Public Economics - - Taxation, Subsidies, and Revenue

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:83:y:2015:i:c:p:345-351. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.