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Escherichia coli hydrogen gas production from glycerol: Effects of external formate

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  • Trchounian, Karen
  • Trchounian, Armen

Abstract

Hydrogen gas (H2) production was studied in bacteria cultured at different pH conditions, and on different carbon substrates. H2 production by Escherichia coli was first studied during glycerol fermentation when external formate (10 mM) was supplemented. Changes in H2 production rate (VH2) were determined when in the assays glycerol and/or formate were supplemented at pH 7.5 and pH 6.5, using single and double mutants coding large subunits of different hydrogenases (Hyd-1, Hyd-2 and Hyd-3) as well as selC (coding for formate dehydrogenases), hyaB hybC (coding for large subunits of Hyd-1 and Hyd-2, respectively) selC or hyaB hybC hycE (coding for large subunit of Hyd-3) triple mutants at both pHs. The results point out that Hyd-3 becomes mainly responsible for H2 production by E. coli during glycerol fermentation when external formate is added; Hyd-4 can also contribute to H2 production. Besides, in the glycerol supplemented assays, three hydrogenases can work in H2 producing mode and only deletion of three of them decreases the production of H2 which might be due to disturbance of H2 cycling. This is of significance in application of different carbon sources in renewable energy production technology using bacteria.

Suggested Citation

  • Trchounian, Karen & Trchounian, Armen, 2015. "Escherichia coli hydrogen gas production from glycerol: Effects of external formate," Renewable Energy, Elsevier, vol. 83(C), pages 345-351.
    • Handle: RePEc:eee:renene:v:83:y:2015:i:c:p:345-351
    DOI: 10.1016/j.renene.2015.04.052
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    References listed on IDEAS

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    1. Gabrielyan, Lilit & Sargsyan, Harutyun & Hakobyan, Lilit & Trchounian, Armen, 2014. "Regulation of hydrogen photoproduction in Rhodobacter sphaeroides batch culture by external oxidizers and reducers," Applied Energy, Elsevier, vol. 131(C), pages 20-25.
    2. Yun Jae Kim & Hyun Sook Lee & Eun Sook Kim & Seung Seob Bae & Jae Kyu Lim & Rie Matsumi & Alexander V. Lebedinsky & Tatyana G. Sokolova & Darya A. Kozhevnikova & Sun-Shin Cha & Sang-Jin Kim & Kae Kyou, 2010. "Formate-driven growth coupled with H2 production," Nature, Nature, vol. 467(7313), pages 352-355, September.
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    Cited by:

    1. Trchounian, Karen & Sawers, R. Gary & Trchounian, Armen, 2017. "Improving biohydrogen productivity by microbial dark- and photo-fermentations: Novel data and future approaches," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 1201-1216.
    2. Trchounian, Karen & Trchounian, Armen, 2015. "Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives," Applied Energy, Elsevier, vol. 156(C), pages 174-184.

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    More about this item

    Keywords

    Escherichia coli; H2 energy; Hydrogenases; Glycerol fermentation; External formate; pH;
    All these keywords.

    JEL classification:

    • H2 - Public Economics - - Taxation, Subsidies, and Revenue

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