IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v83y2015icp1076-1083.html
   My bibliography  Save this article

Genetic engineering of Clostridium acetobutylicum to enhance isopropanol-butanol-ethanol production with an integrated DNA-technology approach

Author

Listed:
  • Bankar, Sandip B.
  • Jurgens, German
  • Survase, Shrikant A.
  • Ojamo, Heikki
  • Granström, Tom

Abstract

Acetone being a non-fuel solvent produced during the traditional acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum; reduces the overall fuel alcohol yield. However, the conversion of acetone into isopropanol has been recommended to improve the process economy. The present study aims to develop an engineered C. acetobutylicum DSM 792 strain to convert acetone into isopropanol by introducing the secondary alcohol dehydrogenase gene from Clostridium beijerinckii NRRL B593 using the allele-coupled exchange approach. Batch and continuous fermentation experiments were carried out with a modified strain C. acetobutylicum DSM 792-ADH to improve the isopropanol yield and titer. The growth and production behavior of the modified strain in stationary flask culture and controlled batch culture was studied. Almost 50% of acetone was converted into isopropanol with highest total solvent yield to be 0.39 g g−1 glucose. The modified strain also utilized sugar mixture and SO2–ethanol–water spent liquor as a substrate to produce the solvents.

Suggested Citation

  • Bankar, Sandip B. & Jurgens, German & Survase, Shrikant A. & Ojamo, Heikki & Granström, Tom, 2015. "Genetic engineering of Clostridium acetobutylicum to enhance isopropanol-butanol-ethanol production with an integrated DNA-technology approach," Renewable Energy, Elsevier, vol. 83(C), pages 1076-1083.
    • Handle: RePEc:eee:renene:v:83:y:2015:i:c:p:1076-1083
    DOI: 10.1016/j.renene.2015.05.052
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S096014811530015X
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2015.05.052?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Shanmugam, Sabarathinam & Ngo, Huu-Hao & Wu, Yi-Rui, 2020. "Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review," Renewable Energy, Elsevier, vol. 149(C), pages 1107-1119.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:83:y:2015:i:c:p:1076-1083. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.