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Optimization of selected salts concentration for improved biohydrogen production from biodiesel-based glycerol using Enterobacter aerogenes

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  • Jitrwung, Rujira
  • Verrett, Jonathan
  • Yargeau, Viviane

Abstract

Enterobacter aerogenes have a known ability to convert glycerol (GL) in a fermentative process to yield hydrogen and ethanol as the main by-products. The concentration of some media constituents was optimized to maximize biohydrogen yield and rate of production. E. aerogenes were cultured in aerobic conditions, and then transferred into anaerobic conditions before being cultured in a minimum mineral synthetic media (MMSM) containing 15 g/L GL. The concentration of selected salts were optimized in the following ranges: 0–300 mg/L MgSO4, 0–14 g/L Na2EDTA, 0–10 mg/L CaCL2, 0–10 g/L Na2HPO4, and 0–9.7 g/L KH2PO4. The results of the full factorial design indicated that the production of biohydrogen required a minimal concentration of 3.5 mg/L EDTA, 200 mg/L MgSO4.7H2O and no CaCl2.2H2O. A significant interaction between EDTA and MgSO4 was also observed. Results from the phosphate salts optimization showed that Na2HPO4 gave better results than KH2PO4. The optimal conditions determined using pure glycerol (commercial grade glycerol), were successfully applied to the fermentation of crude glycerol from biodiesel production. The results indicated promising yields of 0.79 and 0.84 mol/mol of glycerol for bioethanol and biohydrogen, respectively, and this at a faster rate than reported previously for E. aerogenes.

Suggested Citation

  • Jitrwung, Rujira & Verrett, Jonathan & Yargeau, Viviane, 2013. "Optimization of selected salts concentration for improved biohydrogen production from biodiesel-based glycerol using Enterobacter aerogenes," Renewable Energy, Elsevier, vol. 50(C), pages 222-226.
    • Handle: RePEc:eee:renene:v:50:y:2013:i:c:p:222-226
    DOI: 10.1016/j.renene.2012.06.049
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    Cited by:

    1. Amani, H. & Ahmad, Z. & Hameed, B.H., 2014. "Synthesis of fatty acid methyl esters via the methanolysis of palm oil over Ca3.5xZr0.5yAlxO3 mixed oxide catalyst," Renewable Energy, Elsevier, vol. 66(C), pages 680-685.
    2. Pachapur, Vinayak Laxman & Sarma, Saurabh Jyoti & Brar, Satinder Kaur & Le Bihan, Yann & Buelna, Gerardo & Verma, Mausam, 2016. "Surfactant mediated enhanced glycerol uptake and hydrogen production from biodiesel waste using co-culture of Enterobacter aerogenes and Clostridium butyricum," Renewable Energy, Elsevier, vol. 95(C), pages 542-551.
    3. Trchounian, Karen & Trchounian, Armen, 2015. "Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives," Applied Energy, Elsevier, vol. 156(C), pages 174-184.
    4. Mangayil, Rahul & Aho, Tommi & Karp, Matti & Santala, Ville, 2015. "Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components," Renewable Energy, Elsevier, vol. 75(C), pages 583-589.
    5. Seifert, K. & Zagrodnik, R. & Stodolny, M. & Łaniecki, M., 2018. "Biohydrogen production from chewing gum manufacturing residue in a two-step process of dark fermentation and photofermentation," Renewable Energy, Elsevier, vol. 122(C), pages 526-532.

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