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Enhanced methanol tolerance of ZIF-8-immobilized Aspergillus oryzae lipase for biodiesel production from used cooking oil

Author

Listed:
  • Xia, Shuaibo
  • Shen, Cai
  • Lin, Jiale
  • Tu, Maolin
  • Tan, Chin-Ping
  • Cheong, Ling-Zhi

Abstract

The absorption process of Aspergillus oryzae lipase (AOL) onto the surface of ZIF-8 was elucidated using molecular dynamic (MD) simulation. AOL are absorbed onto the surface of ZIF-8 via hydrogen bonding, electrostatic and van der Waals interaction with minimal changes to its native configurations. Unlike free AOL, AOL@ZIF-8 can maintain its structure in simulated methanol solutions. To verify the results from MD simulation, AOL was immobilized onto ZIF-8 (AOL@ZIF-8: immobilization efficiency of 64.71 % and a protein loading of 57.36 mg/g) and characterized using SEM, FTIR, and XRD. As compared to free AOL, AOL@ZIF-8 demonstrated higher transesterification activity with an activity recovery of 106.18 %. In agreement with MD simulations, AOL@ZIF-8 exhibited enhanced stability in wide range of pH and under presence of alcohol solution. AOL@ZIF-8 can be used to catalyze transesterification of used cooking oil for production of biodiesel (FAME content of 81.19 %).

Suggested Citation

  • Xia, Shuaibo & Shen, Cai & Lin, Jiale & Tu, Maolin & Tan, Chin-Ping & Cheong, Ling-Zhi, 2025. "Enhanced methanol tolerance of ZIF-8-immobilized Aspergillus oryzae lipase for biodiesel production from used cooking oil," Renewable Energy, Elsevier, vol. 239(C).
    • Handle: RePEc:eee:renene:v:239:y:2025:i:c:s0960148124021906
    DOI: 10.1016/j.renene.2024.122122
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