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Efficient fermentable sugar production from mulberry branch based on a rational design of GH10 xylanase with improved thermal stability

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  • Bai, Zhi-Yuan
  • You, Shuai
  • Zhang, Fang
  • Dong, Zhi-Wei
  • Zhao, Yi-Fan
  • Wen, Hong-Jian
  • Wang, Jun

Abstract

The mulberry branch can be used as a renewable biomass resource for the production of biofuels since it contains a significant amount of lignocellulose. However, the rapid deactivation of enzymes during lignocellulose degradation in a high-temperature environment leads to high costs and low efficiency of enzymatic saccharification. In this study, xylanase was modified by rational design, and xylanase and cellulase were used to synergistically degrade mulberry branches pretreated with seawater. The fermentable sugar yield and degree of synergy (DS) were determined. The dominant mutant S21Y/N318W of Hortaea werneckii xylanase Hwxyl10A was obtained with improved thermostability (T50 increased by 12 °C, t1/2 is 17-fold that of WT). Meanwhile, the specific activity at 75 °C increased from 1470 to 1901 U/mg. Under the condition of seawater immersion pretreatment, the highest yield of fermentable sugar was 313.5 μmol/g, and the highest DS was 1.57 after enzymatic hydrolysis of mulberry branches by cellulase and S21Y/N318W at 50 °C. Therefore, the biotransformation of mulberry branches reducing sugar has been significantly improved, which provides an efficient method of biomass saccharification for biofuel production.

Suggested Citation

  • Bai, Zhi-Yuan & You, Shuai & Zhang, Fang & Dong, Zhi-Wei & Zhao, Yi-Fan & Wen, Hong-Jian & Wang, Jun, 2023. "Efficient fermentable sugar production from mulberry branch based on a rational design of GH10 xylanase with improved thermal stability," Renewable Energy, Elsevier, vol. 206(C), pages 566-573.
    • Handle: RePEc:eee:renene:v:206:y:2023:i:c:p:566-573
    DOI: 10.1016/j.renene.2023.02.043
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    References listed on IDEAS

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    1. Gottumukkala, Lalitha Devi & Haigh, Kate & Görgens, Johann, 2017. "Trends and advances in conversion of lignocellulosic biomass to biobutanol: Microbes, bioprocesses and industrial viability," Renewable and Sustainable Energy Reviews, Elsevier, vol. 76(C), pages 963-973.
    2. Paz-Cedeno, Fernando Roberto & Henares, Lucas Ragnini & Solorzano-Chavez, Eddyn Gabriel & Scontri, Mateus & Picheli, Flávio Pereira & Miranda Roldán, Ismael Ulises & Monti, Rubens & Conceição de Olive, 2021. "Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads," Renewable Energy, Elsevier, vol. 165(P1), pages 1-13.
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