IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v188y2022icp997-1007.html
   My bibliography  Save this article

Production of polysaccharide bioflocculants and gene co-expression network analysis in a biomass-degrading bacterium, Pseudomonas sp. GO2

Author

Listed:
  • Guo, Haipeng
  • Fu, Xuezhi
  • Chen, Yifan
  • Feng, Jiayin
  • Qi, Zhenyu
  • Yan, Mengchen
  • Zheng, Bingsong
  • Qin, Wensheng
  • Shao, Qingsong

Abstract

Several bacteria exhibit reveal excellent production ability of bioflocculants through directly utilizing untreated biomass. However, mechanisms of bioflocculant synthesis in these bacteria remain to be fully clarified. In this study, a biomass-degrading bacterium Pseudomonas sp. GO2 produced a polysaccharide bioflocculant with the maximal flocculating efficiency of 91.5% when corn stover was used as the sole carbon source. To investigate the molecular mechanism of bioflocculant production, the transcriptome of GO2 strain was measured. Using pairwise comparisons and WGCNA analysis, a total of 1032 differentially expressed genes (DEGs) were successfully divided into four co-expression modules. Among them, one specific module (turquoise) was closely associated with CMCase and xylanase activities, and two modules (brown and yellow) were significantly related to the flocculating efficiency. Finally, DEGs related to seven CAZymes and eight secretory proteins were identified as key candidate genes, and seven potential gene clusters associated with these genes may play important roles in biomass degradation and bioflocculant synthesis in GO2 strain. The results not only enrich genomic resource but also provides a solid theory foundation for the rational synthesis of high efficient and low cost bioflocculants.

Suggested Citation

  • Guo, Haipeng & Fu, Xuezhi & Chen, Yifan & Feng, Jiayin & Qi, Zhenyu & Yan, Mengchen & Zheng, Bingsong & Qin, Wensheng & Shao, Qingsong, 2022. "Production of polysaccharide bioflocculants and gene co-expression network analysis in a biomass-degrading bacterium, Pseudomonas sp. GO2," Renewable Energy, Elsevier, vol. 188(C), pages 997-1007.
    • Handle: RePEc:eee:renene:v:188:y:2022:i:c:p:997-1007
    DOI: 10.1016/j.renene.2022.02.084
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148122002324
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2022.02.084?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Guo, Haipeng & Chen, Houming & Fan, Lu & Linklater, Andrew & Zheng, Bingsong & Jiang, Dean & Qin, Wensheng, 2017. "Enzymes produced by biomass-degrading bacteria can efficiently hydrolyze algal cell walls and facilitate lipid extraction," Renewable Energy, Elsevier, vol. 109(C), pages 195-201.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhang, Yi & Kong, Xiaoying & Wang, Zhongming & Sun, Yongming & Zhu, Shunni & Li, Lianhua & Lv, Pengmei, 2018. "Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp," Renewable Energy, Elsevier, vol. 125(C), pages 1049-1057.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:188:y:2022:i:c:p:997-1007. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.