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Improving cellulase recycling efficiency by decreasing the inhibitory effect of unhydrolyzed solid on recycled corn stover saccharification

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  • Xin, Donglin
  • Yang, Ming
  • Chen, Xiang
  • Zhang, Ying
  • Wang, Rui
  • Wen, Peiyao
  • Zhang, Junhua

Abstract

Recycling enzymes associated with unhydrolyzed solid residue offers a potential strategy to reduce the amount of enzyme used during production of biofuels from lignocellulosic biomass. In this work, the hydrolytic efficiency of the enzymes associated with unhydrolyzed corn stover residue is evaluated and strategies to enhance their recycling efficiency are developed. We found whether direct recycling of corn stover residue could boost the recycling hydrolysis was dependent on the dosage of fresh cellulases. Under the investigated conditions, the addition of 5 FPU/g DM cellulases and BSA (2.5 mg/mL) could take the most advantage of the bound enzymes and suppress the negative effects caused by recalcitrant solid residues. The enzyme loading could be reduced by approximately 50% to achieve the same glucose yields (approximately 85%). This provided a highly promising technology for the optimization of solid residue recycling process and achieved the ultimate goal of cheaper and more efficient hydrolysis of lignocellulosic biomass.

Suggested Citation

  • Xin, Donglin & Yang, Ming & Chen, Xiang & Zhang, Ying & Wang, Rui & Wen, Peiyao & Zhang, Junhua, 2020. "Improving cellulase recycling efficiency by decreasing the inhibitory effect of unhydrolyzed solid on recycled corn stover saccharification," Renewable Energy, Elsevier, vol. 145(C), pages 215-221.
    • Handle: RePEc:eee:renene:v:145:y:2020:i:c:p:215-221
    DOI: 10.1016/j.renene.2019.06.029
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    Cited by:

    1. Wang, Peng & Su, Yan & Tang, Wei & Huang, Caoxing & Lai, Chenhuan & Ling, Zhe & Yong, Qiang, 2022. "Revealing enzymatic digestibility of kraft pretreated larch based on a comprehensive analysis of substrate-related factors," Renewable Energy, Elsevier, vol. 199(C), pages 1461-1468.

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