IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v69y2024i5id106-2023-vetmed.html
   My bibliography  Save this article

Limosilactobacillus reuteri L26 BiocenolTM and its exopolysaccharide: Their influence on rotavirus-induced immune molecules in enterocyte-like cells

Author

Listed:
  • P Schusterova

    (Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic)

  • D Mudronová

    (Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic)

  • K Loziakova Penazziova

    (Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic)

  • V Hajducková

    (Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic)

  • T Csank

    (Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic)

Abstract

This study aimed to evaluate the immunomodulatory effect of the probiotic Limosilactobacillus reuteri L26 BiocenolTM (L26) and its purified exopolysaccharide (EPS) with respect to antiviral innate immune response. In our experiment, we used porcine epithelial IPEC-J2 cells as a model of the intestinal barrier in a homologous infection by porcine Rotavirus A strain OSU6 (RVA). The production of selected molecules of non-specific humoral immunity was evaluated at the mRNA level. The EPS alone significantly increased the level of interferon λ3 (IFN-λ3) mRNA in the non-infected IPEC-J2 cells (P < 0.001). We also tested whether the treatment of IPEC-J2 cells by L26 or EPS influences the replication of RVA by virus titration and real-time PCR. We found that a pre-treatment in combination with subsequent continuous stimulation has no influence on the RVA replication. However, both treatments significantly decreased the RVA-induced production of IFN-λ3 (P < 0.05) and the "SOS" cytokine interleukin 6 (IL-6; P < 0.01), already at the transcription level. In addition, the EPS treatment resulted in significantly increased IL-10 mRNA in the RVA-infected cells. In summary, we assume an immunoregulatory potential of L. reuteri L26 BiocenolTM and its EPS in the local intestinal antiviral immune response.

Suggested Citation

  • P Schusterova & D Mudronová & K Loziakova Penazziova & V Hajducková & T Csank, 2024. "Limosilactobacillus reuteri L26 BiocenolTM and its exopolysaccharide: Their influence on rotavirus-induced immune molecules in enterocyte-like cells," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 69(5), pages 169-176.
    • Handle: RePEc:caa:jnlvet:v:69:y:2024:i:5:id:106-2023-vetmed
    DOI: 10.17221/106/2023-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/106/2023-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/106/2023-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/106/2023-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:69:y:2024:i:5:id:106-2023-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.