IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v67y2022i2id141-2020-vetmed.html
   My bibliography  Save this article

Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-J

Author

Listed:
  • J Yang

    (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China)

  • P Zhuang

    (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China)

  • Z Cheng

    (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China)

  • G Wang

    (Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China)

Abstract

Exploration of the abnormal expression of exosomal molecules during the infection of avian leukosis virus subgroup J (ALV-J) is essential to provide a deeper understanding of the exosome's role in the viral pathogenesis involved. The study aimed to investigate the differentially expressed proteins and miRNAs of the exosomes derived from DF-1 cells infected by ALV-J, their gene function and involved signal pathways. We isolated exosomes from DF-1 cells infected by ALV-J. The differentially expressed proteins and miRNAs of the exosomes were determined by proteomics and transcription detection technology. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and the signal pathways regulated by the different proteins or/and miRNAs. A total of 116 proteins (58 upregulated and 58 downregulated) and 3 miRNAs (all upregulated) were determined. These proteins were involved in 155 signal pathways, in which the highest number of proteins involved in the cancer pathway was (up to) seven. The target genes of the miRNAs were involved in 3 signal pathways. Both the proteins and target genes of the miRNAs were involved in the Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs of the exosomes significantly.

Suggested Citation

  • J Yang & P Zhuang & Z Cheng & G Wang, 2022. "Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-J," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 67(2), pages 87-98.
    • Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:141-2020-vetmed
    DOI: 10.17221/141/2020-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/141/2020-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/141/2020-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/141/2020-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:141-2020-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.