IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v64y2019i5id125-2018-vetmed.html
   My bibliography  Save this article

Cryopreservation and quality assessment of boar semen collected from bulk samples

Author

Listed:
  • Ruthaiporn Ratchamak

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand)

  • Thevin Vongpralub

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
    Thermo-tolerance Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, Thailand)

  • Wuttigrai Boonkum

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
    Thermo-tolerance Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, Thailand)

  • Vibuntita Chankitisakul

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
    Thermo-tolerance Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, Thailand)

Abstract

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P < 0.05) had a lower mean sperm concentration (P < 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P < 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

Suggested Citation

  • Ruthaiporn Ratchamak & Thevin Vongpralub & Wuttigrai Boonkum & Vibuntita Chankitisakul, 2019. "Cryopreservation and quality assessment of boar semen collected from bulk samples," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 64(5), pages 209-216.
    • Handle: RePEc:caa:jnlvet:v:64:y:2019:i:5:id:125-2018-vetmed
    DOI: 10.17221/125/2018-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/125/2018-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/125/2018-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/125/2018-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:64:y:2019:i:5:id:125-2018-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.