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Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA

Author

Listed:
  • J. Sanchis

    (Parasitology Department, University of República (Regional Norte), Salto, Uruguay)

  • J. Suarez

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • G.V. Hillyer

    (School of Medicine, University of Puerto Rico, San Juan, USA)

  • J.A. Hernandez

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • M.A. Solari

    (Parasitology Department, Veterinary Laboratories Division (DILAVE), Uruguay)

  • C. Cazapal-Monteiro

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • A.M. Duque de Araujo

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • L.M. Madeira de Carvalho

    (Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal)

  • A. Paz-Silva

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • R. Sanchez-Andrade

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

  • M.S. Arias

    (Veterinary Faculty, Santiago de Compostela University, Lugo, Spain)

Abstract

The risk of exposure to Fasciola hepatica in horses from Uruguay was evaluated using ELISA and a recombinant surface protein (FhrAPS). Blood samples were collected from 368 horses from different districts. Detection of antibodies and the seasonal IgG pattern against the trematode was also established. The overall seroprevalence was 54% (ranging 23% November to 93% December). Significantly higher values were observed in the Anglo-Arab horses (86%) and in those older than xix year (63%). No sex- or origin-differences were observed. Two primary risk periods for infection in Uruguay have been identified, the end of spring and autumn. Exposure to the fluke should be taken into account among those horses feeding on pastures grazed by ruminants. Using FhrAPS-ELISA, helpful information concerning the risk of horses to the infection by trematode can be obtained. This test allows the opportunity to compare the results achieved in different laboratories by minimising specific regional effects.

Suggested Citation

  • J. Sanchis & J. Suarez & G.V. Hillyer & J.A. Hernandez & M.A. Solari & C. Cazapal-Monteiro & A.M. Duque de Araujo & L.M. Madeira de Carvalho & A. Paz-Silva & R. Sanchez-Andrade & M.S. Arias, 2015. "Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 60(9), pages 483-488.
    • Handle: RePEc:caa:jnlvet:v:60:y:2015:i:9:id:8439-vetmed
    DOI: 10.17221/8439-VETMED
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