IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v58y2013i11id7138-vetmed.html
   My bibliography  Save this article

Prevalence of mastitis pathogens in milk from clinically healthy cows

Author

Listed:
  • D. Cervinkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • H. Vlkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Borodacova

    (Veterinary Research Institute, Brno, Czech Republic)

  • J. Makovcova

    (Veterinary Research Institute, Brno, Czech Republic)

  • V. Babak

    (Veterinary Research Institute, Brno, Czech Republic)

  • A. Lorencova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Vrtkova

    (Laboratory of Agrogenomics, Mendel University, Brno, Czech Republic)

  • D. Marosevic

    (Veterinary Research Institute, Brno, Czech Republic)

  • Z. Jaglic

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negative staphylococci clearly predominated (53.5% positive samples) followed by streptococci and enterococci (both occurring in 16.1% samples). Among streptococci, so-called mastitis streptococci (S. uberis, S. dysgalactiae and S. agalactiae) prevailed (11.7% positive samples). Enterobacteriaceae were found in 10.0% samples, most of which (6.6% samples) were positive for Escherichia coli. Yeasts (mainly Candida spp.) were found in 8.2% samples. One of the major mastitis pathogens, Staphylococcus aureus subsp. aureus, was isolated from 9.0% of samples. S. aureus isolates were further characterised in terms of their capability to form biofilm, antimicrobial susceptibility and clonality (PFGE). All S. aureus isolates were capable of biofilm formation and were generally susceptible to the majority of tested antibiotics. The exception was ampicillin, resistance to which was observed in 27.7% isolates. Therefore, the relatively frequent occurrence of S. aureus could be attributed to persistent intramammary infections due to biofilm formation rather than low efficacy of particular antibiotics. PFGE analysis revealed clonal spread of certain S. aureus isolates within and between farms indicating that certain lineages of S. aureus mastitis strains are particularly successful.

Suggested Citation

  • D. Cervinkova & H. Vlkova & I. Borodacova & J. Makovcova & V. Babak & A. Lorencova & I. Vrtkova & D. Marosevic & Z. Jaglic, 2013. "Prevalence of mastitis pathogens in milk from clinically healthy cows," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 58(11), pages 567-575.
    • Handle: RePEc:caa:jnlvet:v:58:y:2013:i:11:id:7138-vetmed
    DOI: 10.17221/7138-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7138-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7138-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/7138-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. D. Rysanek & V. Babak & M. Zouharova, 2007. "Bulk tank milk somatic cell count and sources of raw milk contamination with mastitis pathogens," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(6), pages 223-230.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. P. Zajac & S. Zubricka & J. Capla & L. Zelenakova, 2016. "Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 61(11), pages 612-622.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. P. Zajac & S. Zubricka & J. Capla & L. Zelenakova, 2016. "Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 61(11), pages 612-622.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:58:y:2013:i:11:id:7138-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.