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Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran

Author

Listed:
  • H. Momtaz

    (Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran)

  • E. Rahimi

    (Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran)

  • S. Moshkelani

    (Young Researchers Club, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran)

Abstract

This study was carried out to detect the distribution of antibiotic-resistant genes in Escherichia coli isolates from slaughtered commercial chickens in Iran by PCR. The investigated genes included aadA1, tet(A), tet(B), dfrA1, qnrA, aac(3)-IV, sul1, blaSHV, blaCMY, ere(A), catA1 and cmlA. According to biochemical experiments, 57 isolates from 360 chicken meat samples were recognized as E. coli. The distribution of antibiotic-resistance genes in the E. coli isolates included tet(A) and tet(B) (52.63%), dfrA1, qnrA, catA1 and cmlA (36.84%) and sul1 and ere(A) (47.36%), respectively. Nine strains (15.78%) were resistant to a single antimicrobial agent and 11 strains (19.29%) showed resistance to two antimicrobial agents. Multi-resistance which was defined as resistance to three or more tested agents was found in 64.91% of E. coli strains. The results indicate that all isolates harbour one or more of antibiotic resistance genes and that the PCR technique is a fast, practical and appropriate method for determining the presence of antibiotic-resistance genes.

Suggested Citation

  • H. Momtaz & E. Rahimi & S. Moshkelani, 2012. "Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(4), pages 193-197.
    • Handle: RePEc:caa:jnlvet:v:57:y:2012:i:4:id:5916-vetmed
    DOI: 10.17221/5916-VETMED
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    Cited by:

    1. S.N. Gharajalar & M. Hassanzade, 2017. "Antibacterial properties of Carum copticum essential oil against Streptococcus mutans and Streptococcus sobrinus isolated from canine dental plaque," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 62(12), pages 654-660.

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