IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v56y2011i10id3295-vetmed.html
   My bibliography  Save this article

DNA damage of blood lymphocytes and neutrophils in cattle with lymphosarcoma

Author

Listed:
  • T. Mohamed

    (Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt)

  • D. Endoh

    (School of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midorimachi, Ebetsu, Hokkaido, Japan)

  • S. Oikawa

    (School of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midorimachi, Ebetsu, Hokkaido, Japan)

Abstract

The objective of the present study was to analyze the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in cows clinically affected with lymphosarcoma. Thirteen cows were studied. Of them, eight, that were referred because of inappetance, loss of body condition, diarrhoea, constipation, protrusion of third eyelid, and exophthalmia, were seropositive for bovine leukemia virus (BLV) based on a serum enzyme-linked immunosorbent assay. Other animals were apparently healthy and were used as controls. DNA damage of PBMC and PMN was assessed using the Comet assay. The results obtained showed a statistically significant difference in DNA damage between the PBMC and PMN isolated from cows infected with BLV compared to PBMC and PMN isolated from healthy cows. This is the first article to document decreased apoptosis of blood PBMC and PMN in cattle in response to BLV infection using the Comet assay.

Suggested Citation

  • T. Mohamed & D. Endoh & S. Oikawa, 2011. "DNA damage of blood lymphocytes and neutrophils in cattle with lymphosarcoma," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 56(10), pages 504-509.
    • Handle: RePEc:caa:jnlvet:v:56:y:2011:i:10:id:3295-vetmed
    DOI: 10.17221/3295-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3295-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3295-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/3295-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:56:y:2011:i:10:id:3295-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.