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Lactoferrin inhibits E. coli O157:H7 growth and attachment to intestinal epithelial cells

Author

Listed:
  • M. Atef Yekta

    (Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium)

  • F. Verdonck

    (Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium)

  • W. Van Den Broeck

    (Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium)

  • B.M. Goddeeris

    (Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium
    Faculty of Bioscience Engineering, Catholic University, Leuven, Belgium)

  • E. Cox

    (Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium)

  • D. Vanrompay

    (Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocarcinoma cell line (Caco-2). The direct antibacterial effect of bLF on E. coli O157:H7 was stronger than that of hLF. Nevertheless, both lactoferrins had bacteriostatic effects even at high concentrations (10 mg/ml), suggesting blocking of LF activity by a yet undefined bacterial defence mechanism. Additionally, both lactoferrins significantly inhibited E. coli O157:H7 attachment to Caco-2 cells. However, hLF was more effective than bLF, probably due to more efficient binding of bLF to intelectin present on human enterocytes leading to uptake and thus removal of bLF from the extracellular environment. Inhibition of bacterial attachment to Caco-2 cells was at least partly due to the catalytic effect of lactoferrins on the type III secreted proteins EspA and EspB

Suggested Citation

  • M. Atef Yekta & F. Verdonck & W. Van Den Broeck & B.M. Goddeeris & E. Cox & D. Vanrompay, 2010. "Lactoferrin inhibits E. coli O157:H7 growth and attachment to intestinal epithelial cells," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(8), pages 359-368.
    • Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2954-vetmed
    DOI: 10.17221/2954-VETMED
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