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Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells

Author

Listed:
  • M.K. Kowalik

    (Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland)

  • D. Slonina

    (Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland)

  • J. Kotwica

    (Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland)

Abstract

Progesterone (P2 (PGES), (b) protein expression of COX-2, PGFS and PGES, and (c) P4 receptor membrane component 1 (PGRMC1) gene expression in bovine endometrial cells. The epithelial endometrial cells (2.5 × 105/ml) from Days 14-16 of the oestrous cycle were incubated for 72-96 h to attach the cells to the bottom of a well. Next, the cells were preincubated for 30 min with P4 and P5 (10-5M each) and incubated for 4 h and 6 h alone or with OT (10-7M). Thereafter, the medium was collected for PGE2 and PGFM determination, while cells were harvested for gene and protein expression analysis. The used steroids: (a) inhibited OT-stimulated PGF2α, but not PGE2 secretion from endometrial cells, (b) did not affect the expression of mRNA for COX-2, PGFS, PGES and PGRMC1 in endometrial cells after 4 and 6 h, (c) they decreased OT-stimulated COX-2 mRNA expression only after 6 h incubation, and (d) did not influence COX-2, PGFS and PGES protein expression after 6 h. These results indicate that P4 and P5 inhibit OT-stimulated secretion/production of luteolytic PGF2α by a transcription-independent mechanism and partly by down-regulation of COX-2 mRNA.

Suggested Citation

  • M.K. Kowalik & D. Slonina & J. Kotwica, 2009. "Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(5), pages 205-214.
    • Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:58-2009-vetmed
    DOI: 10.17221/58/2009-VETMED
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    References listed on IDEAS

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    1. Eric Grazzini & Gilles Guillon & Bernard Mouillac & Hans H. Zingg, 1998. "Inhibition of oxytocin receptor function by direct binding of progesterone," Nature, Nature, vol. 392(6675), pages 509-512, April.
    2. R. Rekawiecki & J. Kotwica, 2007. "Molecular regulation of progesterone synthesis in the bovine corpus luteum," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(9), pages 405-412.
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