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Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

Author

Listed:
  • D.Y. Zhang

    (College of Biology and Environmental Eengineering, Zhejiang Shuren University, Hangzhou, China)

  • J.Y. Zhou

    (Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China)

  • W.Q. Chen

    (College of Biology and Environmental Eengineering, Zhejiang Shuren University, Hangzhou, China)

  • J.G. Chen

    (Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China
    College of Biological and Environmental Engineering, Zhejiang Wanli University, Ningbo, China)

Abstract

The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocytochemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection.

Suggested Citation

  • D.Y. Zhang & J.Y. Zhou & W.Q. Chen & J.G. Chen, 2009. "Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(4), pages 169-174.
    • Handle: RePEc:caa:jnlvet:v:54:y:2009:i:4:id:23-2009-vetmed
    DOI: 10.17221/23/2009-VETMED
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    References listed on IDEAS

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    1. D.Y. Zhang & J.Y. Zhou & J. Fang & J.Q. HU & J.X. WU & A.X. MU, 2005. "An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(8), pages 336-344.
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      Keywords

      IBV; S1 gene; IL2; expression;
      All these keywords.

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