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Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

Author

Listed:
  • R. Smitalova

    (Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic)

  • L. Rodak

    (Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic)

  • B. Smid

    (Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic)

  • I. Psikal

    (Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic)

Abstract

Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain.

Suggested Citation

  • R. Smitalova & L. Rodak & B. Smid & I. Psikal, 2009. "Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(1), pages 12-18.
    • Handle: RePEc:caa:jnlvet:v:54:y:2009:i:1:id:3081-vetmed
    DOI: 10.17221/3081-VETMED
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    1. R. Smitalova & L. Rodak & I. Psikal & B. Smid, 2006. "Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 288-295.
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