Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea

The porcine enteroviruses (family Picornaviridae) were described as causative agents of neurological disorders known as Teschen/Talfan disease, reproductive failure, dermal lesions, enteric disease and pneumonia of swine. Recently, porcine enteroviruses were reclassified on the basis of genome sequencing data. A new Picornavirus genus named Teschovirus (formerly PEV CPE group I.) was established. The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms (CAPM). Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 (PEV-1) based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschoviru and the Porcine teschovirus serotype 1 (PTV-1). Two PCR amplifications with cDNA were performed for detection of the genus Teschovirus and PTV-1. Amplification of fragment characteristic for the genus Teschovirus was succesful for all tested viral strains. The fragment characteristic for PTV-1 was detected with the exception of three strains (Kr69TK, 95 and 172). Used RT-PCR method was subsequently applied to the detection of Porcine teschovirus in pig fecal samples. Ten of 22 faecal samples were found to be Porcine teschovirus positive, however none of 22 samples generated amplicon specific for the PTV-1.