IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v52y2007i8id1876-vetmed.html
   My bibliography  Save this article

Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea

Author

Listed:
  • S.N. Sauter

    (Institute of Animal Genetics, Nutrition and Housing, University of Bern, Bern, Switzerland)

  • K. Allenspach

    (Department of Clinical Veterinary Medicine, University of Bern, Bern, Switzerland)

  • J.W. Blum

    (Institute of Animal Genetics, Nutrition and Housing, University of Bern, Bern, Switzerland)

Abstract

The differentiation between inflammatory bowel disease (IBD) and food responsive diarrhea (FRD) is difficult and no objective markers are available. We postulated that patterns of selected key cytokines would help to objectively differentiate between the two subcategories of chronic enteropathies in dogs. We studied mRNA patterns of selected cytokines in dogs with chronic enteropathies. Ten dogs with FRD (= group FRDbef) and seven dogs with IBD (= group IBDbef) were presented for endoscopy at the Small Animal Clinic, University of Bern. A control endoscopy was performed in both groups after treatment with an elimination diet for four weeks (FRDaft) or with an elimination diet combined with prednisolone for 10 weeks (IBDaft). Intestinal control samples of gastrointestinally healthy dogs from an independent study were additionally available. Dogs were clinically examined and scored using the canine IBD activity index (CIBDAI). mRNA abundance of interleukin (IL)-5, -10, -12p40, and -13, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, and interferon (IFN)-γ were analyzed in intestinal samples by reverse transcription and real time polymerase chain reaction. Median CIBDAI decreased in FRDaft (P < 0.01) and IBDaft (P = 0.07) during treatment. In duodenum, IL-12p40 mRNA levels tended to be lower in FRDbef than in IBDbef (P = 0.07). The abundance of TNF-α mRNA was higher in IBDbef than in control dogs (P < 0.05). IL-5 mRNA levels decreased in FRD dogs during treatment (P = 0.06), and IL-10 mRNA levels decreased in IBD dogs (P < 0.05). In colon, IL-5 and IL-12p40 mRNA levels were lower in FRDbef than in IBDbef (P < 0.05) and control dogs (P < 0.01). IL-13 mRNA abundance was lower in FRDbef than in control dogs (P < 0.05) and IFN-γ mRNA abundance was lower in FRD and IBD dogs than in control dogs (P < 0.01). Feeding the elimination diet additionally reduced IFN-γ mRNA levels (P < 0.01), but increased TNF-α mRNA levels (P < 0.05) in FRD dogs. In conclusion, mRNA levels of the selected cytokines before treatment did not show clear differences between FRD and IBD dogs.

Suggested Citation

  • S.N. Sauter & K. Allenspach & J.W. Blum, 2007. "Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(8), pages 353-364.
    • Handle: RePEc:caa:jnlvet:v:52:y:2007:i:8:id:1876-vetmed
    DOI: 10.17221/1876-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1876-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1876-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/1876-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. A.C. Spichiger & K. Allenspach & Y. Zbinden & M.G. Doherr & S. Hiss & J.W. Blum & S.N. Sauter, 2006. "Plasma insulin-like growth factor-1 concentration in dogs with chronic enteropathies," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(1), pages 35-43.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:52:y:2007:i:8:id:1876-vetmed. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.