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Development of an ELISA-based kit for the on-farm determination of progesterone in milk

Author

Listed:
  • R. Simersky

    (Laboratory of Growth Regulators, Palacky University, Olomouc, Czech Republic
    Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • J. Swaczynova

    (Laboratory of Growth Regulators, Palacky University, Olomouc, Czech Republic
    Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • D.A. Morris

    (Laboratory of Growth Regulators, Palacky University, Olomouc, Czech Republic
    Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • M. Franek

    (Veterinary Research Institute, Brno, Czech Republic.)

  • M. Strnad

    (Laboratory of Growth Regulators, Palacky University, Olomouc, Czech Republic
    Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

Abstract

Effective reproductive management is a crucial concern in the dairy industry and missed oestrus and late recognition of unsuccessful insemination can lead to substantial financial loss. Progesterone levels change in a predictable manner with progress of the oestrus cycle and with the onset of pregnancy. We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk. The method is based on the use of polyclonal antibodies raised against 11α-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine. The method has proved to be a cost effective, sensitive and easy to perform alternative to currently available methods of oestrus and pregnancy detection. The practical feasibility of the method has been proven under dairy farm conditions.

Suggested Citation

  • R. Simersky & J. Swaczynova & D.A. Morris & M. Franek & M. Strnad, 2007. "Development of an ELISA-based kit for the on-farm determination of progesterone in milk," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(1), pages 19-28.
    • Handle: RePEc:caa:jnlvet:v:52:y:2007:i:1:id:2009-vetmed
    DOI: 10.17221/2009-VETMED
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