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Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves

Author

Listed:
  • S. Fleige

    (Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany)

  • W. PREIßINGER

    (Institute of Animal Nutrition and Feed Management, Bavarian Regional Office for Agriculture, Poing-Grub, Germany)

  • H.H.D. Meyer

    (Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany)

  • M.W. Pfaffl

    (Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany)

Abstract

The study was conducted to elucidate the effects of orally administered lactulose in combination with Enterococcus faecium on immune response of the intestinal tract in pre-ruminant calves. The mRNA expression of pro- and anti-inflammatory cytokines and proliferation and apoptosis markers were investigated in jejunum, ileum, colon and caecum. Simmental calves were fed diets containing 1% (L1) or 3% (L3) lactulose and the probiotic strain of the genus E. faecium, and compared with a non treated control group. Primarily the high dose feeding with lactulose showed an effect on several mRNA gene expression parameters. In the jejunum a down-regulation of the anti-apoptotic marker Bcl-xl was determined and IL-10 mRNA gene expression was 2.6-fold up-regulated (P < 0.05). In the colon a 1.9-fold (P < 0.05) up-regulation of IL-10 and only in caecum an about 2-fold increase of TGF-β1 (P < 0.05) was found for both lactulose feedings. Caspase 3 was up-regulated in caecum only in the 3% lactulose treated group (P < 0.05). The enhanced apoptotic rate of caspase 3 seems to be associated with a decrease in crypth depth due to lactulose supplementation. The results indicated that mainly the high 3% lactulose dose in probiotic-fed calves has an affect on the intestinal immune function and on diverse apoptotic markers.

Suggested Citation

  • S. Fleige & W. PREIßINGER & H.H.D. Meyer & M.W. Pfaffl, 2007. "Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(10), pages 437-444.
    • Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2046-vetmed
    DOI: 10.17221/2046-VETMED
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    Keywords

    intestine; morphology; health;
    All these keywords.

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