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The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils

Author

Listed:
  • Z. Sládek

    (Mendel University of Agriculture and Forestry, Brno, Czech Republic)

  • D. Vašíčková

    (Mendel University of Agriculture and Forestry, Brno, Czech Republic)

  • D. Ryšánek

    (Mendel University of Agriculture and Forestry, Brno, Czech Republic)

Abstract

The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop­tosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils were already observed in the mammary gland lavages (11.9% and 0.8%, respectively). During the cultivation, a progressive increase in the number of apoptotic neutrophils in various stages of apoptosis - karyopyknosis, zeiosis and apoptotic bodies - was observed. Karyopyknotic neutrophils represented a dominant part of the apoptotic neutrophil population in the course of the whole cultivation. The most intensive increase was observed in zeiosis, whereas the levels of apoptotic bodies remained the same. After 4 hours of cultivation, 31.7% apoptotic neutrophils and 9.8% trypan blue positive neutrophils (i.e. Secondary necrotic cells) were found. The results of this work show that spontaneous apoptosis and secondary neutrophil necrosis must be taken into account during in vitro cultivations of bovine mammary gland neutrophils.

Suggested Citation

  • Z. Sládek & D. Vašíčková & D. Ryšánek, 2002. "The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(12), pages 325-332.
    • Handle: RePEc:caa:jnlvet:v:47:y:2002:i:12:id:5843-vetmed
    DOI: 10.17221/5843-VETMED
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    Citations

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    Cited by:

    1. Z. Sladek & D. Rysanek & M. Faldyna, 2005. "Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland neutrophils in vitro," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(1), pages 11-23.
    2. D. Rysanek & Z. Sladek & V. Babak & D. Vasickova & M. Hubackova, 2006. "Spontaneous and induced cytolysis of leukocytes from bovine mammary gland in the course of cultivation in vitro - the correlation with neutrophil granulocytes apoptosis," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 265-277.

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