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Effects of some antibiotics on glucose 6-phosphate dehydrogenase in sheep liver

Author

Listed:
  • M. Çiftci

    (Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey)

  • V. Turkoglu

    (Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey)

  • S. Aldemir

    (Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey)

Abstract

In vitro effects of penicillin, sulbactam, cefazolin, and amikacine on the activity of the enzyme glucose-6-phosphate dehydrogenase in sheep liver were investigated. Glucose 6-phosphate dehydrogenase was purified from sheep liver, using a simple and rapid method. The purification consisted of two steps, preparation of homogenate and 2', 5'-ADP Sepharose 4B affinity chromatography. As a result of the two consecutive procedures, the enzyme, having the specific activity of 11.76 EU/mg proteins, was purified with a yield of 35.72% and 1.913 fold. In order to control the enzyme purification SDS polyacrylamide gel electrophoresis (SDS-PAGE) was done. SDS-PAGE showed a single band for the enzyme. In addition, I50 values of the antibiotics were determined by plotting activity % vs. antibiotic concentrations. I50 values were 17.71 mM for penicillin, 27.38 mM for sulbactam, 28.88 mM for cefazolin, and 30.59 mM for amikacine.

Suggested Citation

  • M. Çiftci & V. Turkoglu & S. Aldemir, 2002. "Effects of some antibiotics on glucose 6-phosphate dehydrogenase in sheep liver," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(10-11), pages 283-288.
    • Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5836-vetmed
    DOI: 10.17221/5836-VETMED
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    Cited by:

    1. M. Erat & H. Sakiroglu & M. Ciftci, 2003. "Effects of some antibiotics on glutathione reductase from bovine erythrocytes," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(11), pages 305-312.

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