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Extremely low frequency electromagnetic field generator suitable for plant in vitro studies

Author

Listed:
  • Riry Prihatini

    (Indonesian Tropical Fruit Research Institute, Solok, Indonesia)

  • Mohamad Puad Abdullah

    (Department of Cell and Molecular Biology, Faculty Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor DE, Malaysia)

  • Tuan Abdul Rashid bin Tuan Abdullah

    (Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia)

  • Ismail Said

    (Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia)

  • Halil Hussin

    (Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia)

  • Norihan Mohamad Saleh

    (Department of Cell and Molecular Biology, Faculty Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor DE, Malaysia
    Agro-Biotechnology Institute Malaysia, National Institute of Biotechnology Malaysia, Selangor DE, Malaysia)

Abstract

The extremely low frequency electromagnetic field (ELF-EMF) occurs naturally from the earth and artificially as a human invention. The objectives of this study were to develop a suitable ELF-EMF generator for in vitro plants culture studies and to determine the effect of ELF-EMF exposure on in vitro tobacco (Nicotiana tabacum) growth and chlorophyll content. An ELF-EMF generator, the coGEM 1,000 was constructed using four coils of copper wires that were connected to a transformer, multimeter and rheostat. The coGEM 1,000 suitable for tissue culture plants is able to produce stable and uniform 6 and 12 mT 50Hz ELF-EMF in the four coils of the ELF-EMF generator. The tobacco in vitro plantlets were exposed to 6 and 12 mT of 50 Hz ELF-EMF for a period of 0.5, 1, 2 and 4 hours. The exposure to 12 mT ELF-EMF for an hour increased plant growth (shoot height); whereas the exposure to 6 mT Elf-EMF for an hour increased chlorophyll a, chlorophyll b and the total chlorophyll content.

Suggested Citation

  • Riry Prihatini & Mohamad Puad Abdullah & Tuan Abdul Rashid bin Tuan Abdullah & Ismail Said & Halil Hussin & Norihan Mohamad Saleh, 2017. "Extremely low frequency electromagnetic field generator suitable for plant in vitro studies," Research in Agricultural Engineering, Czech Academy of Agricultural Sciences, vol. 63(4), pages 180-186.
    • Handle: RePEc:caa:jnlrae:v:63:y:2017:i:4:id:47-2016-rae
    DOI: 10.17221/47/2016-RAE
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    References listed on IDEAS

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    1. Zhongfu Ni & Eun-Deok Kim & Misook Ha & Erika Lackey & Jianxin Liu & Yirong Zhang & Qixin Sun & Z. Jeffrey Chen, 2009. "Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids," Nature, Nature, vol. 457(7227), pages 327-331, January.
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