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The effect of benzothiazolium salt on spruce callus cells

Author

Listed:
  • Ľ. Krajnáková

    (Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
    NovaMed Policlinic, Banská Bystrica, Slovakia)

  • D. Kákoniová

    (Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia)

  • D. Lišková

    (Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia)

  • E. Hlinková

    (Institute of Cell Biology, Faculty of Natural Sciences, Comenius University,)

Abstract

The effect of 3-(benzyloxycarbonylmethyl)-2-benzothiazolinone (SM-550) on the growth parameters, cell division, and cell polymorphism of spruce callus cells (Picea abies L. Karst) was investigated. These results were compared with callus parameters grown on the medium supplemented with NAA. The highest concentration (1 mmol) of SM-550 stimulated the growth process, as well as cell division, shortened the lag-phase, and had a significant effect on cells polymorphism. Its effect was demonstrated especially on long-term culture (3 subcultures - 84 days). On the other hand, the highest stimulation of growth by SM-550 in 1 μmol concentration was determined only in the first subculture. SM-550 in the lowest concentration (1 nmol) used was completely unsuitable in the third subculture, the callus was necrotic and resembled to calli growing on the medium without growth hormones.

Suggested Citation

  • Ľ. Krajnáková & D. Kákoniová & D. Lišková & E. Hlinková, 2010. "The effect of benzothiazolium salt on spruce callus cells," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 56(10), pages 463-469.
    • Handle: RePEc:caa:jnlpse:v:56:y:2010:i:10:id:149-2009-pse
    DOI: 10.17221/149/2009-PSE
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    References listed on IDEAS

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    1. M. Henselová & A. Lux & E. Masarovičová, 2002. "Effect of growth regulators on rooting cuttings of Karwinskia species under in vivo conditions," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 48(10), pages 471-476.
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