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Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community

Author

Listed:
  • J. Kopecký

    (Crop Research Institute, Prague, Czech Republic)

  • G. Novotná

    (Crop Research Institute, Prague, Czech Republic)

  • M. Ságová-Marečková

    (Crop Research Institute, Prague, Czech Republic)

Abstract

A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.

Suggested Citation

  • J. Kopecký & G. Novotná & M. Ságová-Marečková, 2009. "Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 55(9), pages 397-403.
    • Handle: RePEc:caa:jnlpse:v:55:y:2009:i:9:id:1012-pse
    DOI: 10.17221/1012-PSE
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