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Isolation and characterization of ardicrenin from Ardisia crenata Sims

Author

Listed:
  • Yuan Ma

    (Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, P.R. China)

  • Shangrao Pu

    (Department of Resource and Environment, Sichuan Agriculture University, Dujiangyan, P.R. China)

  • Qingsu Cheng

    (Department of Pharmacy and Bioengineering, Chemical School, Sichuan University, Chengdu, P.R. China)

  • Mingdong Ma

    (Department of Resource and Environment, Sichuan Agriculture University, Dujiangyan, P.R. China)

Abstract

A new, effective and economical method to extract ardicrenin from Ardisia crenata Sims collected in the Wolong natural reserve, Sichuan, China, is established. Ardisia crenata Sims powder is counter-current extracted with 80% methanol reflux, decompressively enriched and centrifuged to defat. Supernatant is applied to macroporous resin column (AB-8) with 80% methanol, ardicrenin is isolated by silica gel chromatography with dichlormethane-acetoacetate-methanol (4:1.5:1) washing, and recrystallized in methanol. The final product which proved to be ardicrenin by analytic procedure including Furier transform infrared (FTIR) and ultraviolet spectrum (UV), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) is white amorphous powder with yield of 1.59 ± 0.02%.

Suggested Citation

  • Yuan Ma & Shangrao Pu & Qingsu Cheng & Mingdong Ma, 2009. "Isolation and characterization of ardicrenin from Ardisia crenata Sims," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 55(7), pages 305-310.
    • Handle: RePEc:caa:jnlpse:v:55:y:2009:i:7:id:62-2009-pse
    DOI: 10.17221/62/2009-PSE
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