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Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

Author

Listed:
  • H. Šimková

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • J. Janda

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • E. Hřibová

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • J. Šafář

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

  • J. Doležel

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic)

Abstract

Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches - i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library - was tested in this work.

Suggested Citation

  • H. Šimková & J. Janda & E. Hřibová & J. Šafář & J. Doležel, 2007. "Cot-based cloning and sequencing of the short arm of wheat chromosome 1B," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 53(10), pages 437-441.
    • Handle: RePEc:caa:jnlpse:v:53:y:2007:i:10:id:2195-pse
    DOI: 10.17221/2195-PSE
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