Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice's calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.