IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpse/v50y2004i4id4075-pse.html
   My bibliography  Save this article

Isolation of chloroplasts in the Karwinskia species and determination of their photochemical activity under in vitro conditions

Author

Listed:
  • M. Henselová

    (Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia)

  • M. Regecová

    (Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia)

  • A. Sováková

    (Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia)

Abstract

Reaction conditions for evaluation of the photochemical activity of isolated chloroplasts in the Hill reaction of Karwinskia humboldtiana (Roem & Schut) Zucc. and Karwinskia parvifolia Rose species were determined. Hill's reaction activity was measured spectrophotometrically at 630 nm as the amount of DCPIP reduction by the chloroplast suspension at an irradiance of 400 µmol/m2/sPAR. A significant difference was observed between the activity of chloroplasts isolated at 2°C and 25°C amounting to 27% in Karwinskia humboldtiana and 18.5% in K. parvifolia. After 24 hours of storage of chloroplasts at a suspension of 2°C, a significant decrease of chloroplasts activity was noted in both species, e.g. 38% in Karwinskia humboldtiana and 45% in K. parvifolia. The photochemical activity of chloroplasts increased also with the length of irradiation of the reaction mixture and the content of chlorophyll (a + b) in chloroplast suspension. The activity of chloroplasts was found to be significantly higher in the species K. humboldtiana than in K. parvifolia and it proved higher in both when these were grown under field conditions rather than in a greenhouse.

Suggested Citation

  • M. Henselová & M. Regecová & A. Sováková, 2004. "Isolation of chloroplasts in the Karwinskia species and determination of their photochemical activity under in vitro conditions," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 50(4), pages 149-156.
    • Handle: RePEc:caa:jnlpse:v:50:y:2004:i:4:id:4075-pse
    DOI: 10.17221/4075-PSE
    as

    Download full text from publisher

    File URL: http://pse.agriculturejournals.cz/doi/10.17221/4075-PSE.html
    Download Restriction: free of charge
    File URL: http://pse.agriculturejournals.cz/doi/10.17221/4075-PSE.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/4075-PSE?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpse:v:50:y:2004:i:4:id:4075-pse. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.